- 期刊
市售人參及西洋參藥材之鑑別
Identification of Panax ginseng C. A. Meyer and Panax quinquefolium L. in Market
摘要
人參(Panax ginseng C. A. Meyer)與西洋參(Panax quinquefolium L.)同屬五加科人參屬植物，其植物型態、藥材性狀、所含化學成分相似，不易鑑別，而野生西洋參之市場價格高於人參數倍，導致市場誤用、偽造情形嚴重，為了解市售人參及西洋參之基原及成分差別，本計畫價購全國北、中、南區中藥廠及中藥房人參藥村46件及西洋參藥村47件，參照文獻之記載，經性狀特徵、組織鏡檢、薄層層析法、PCR-DNA定序方法及極致效能液相層析法鑑別，結果顯示人參46件檢體均為正品，西洋參中藥廠23件檢體中，含人參9件(39%)；中藥房24件檢體中，含人參2件(8%)，整體而言，47件檢體中，正品佔77%，非正品佔23%，市售西洋參誤用情形嚴重，應詳加鑑別。 本實驗之極致效能液相層析法係採用Acquity BEH C18，2.1mm×100 mm，1.7μm管柱，流動相為乙腈－水溶液，利用線性梯度沖提，檢測波長為203 nm，快速檢測人參及西洋參藥村中4種皂苷類成分Ginsenoside R(下標 g1), Ginsenoside R(下標 e), Ginsenoside R(下標 f), Ginsenoside R(下標 b1)之有無。
並列摘要
Panax ginseng C. A. Meyer and P. quinquefolium L. (Araliaceae) are widely used in Chinese medicine as a remedy for a long time. Because of the morphological similarities and analogous chemical composition of Panax species, alternatives and adulterants were found and reported in literatures. In order to identify the botanical origins of commercial Ginseng Radix (P. ginseng) and Panacis Quinquefolii Radix (P. quinquefolium) in Taiwan, 93 samples, purchased from market, were distinguished from authentic materials by morphology, microscopy, TLC (thin layer chromatography), UPLC (Ultra Performance Liquid Chromatography) and PCRDNA sequencing. The results showed that 11 (23%) out of 47 Panacis Quinquefolii Radix samples were P. ginseng, all of 46 Ginseng Radix samples (100%) were correct. Based on the results, the adulteration of Panacis Quinquefolii Radix in market is very serious.