The objective of this study was to assess the effects of various media and trays on the seedlings quality of purple coneflower. These results indicated that the seedling weight in 5 week-age cultured in 60-cell plug tray with Taichung DAIS No 1. medium (M0T1) had positive effect. The fresh weight and dry weight of shoot and root were 1.31 g, 1.569 g, 0.169 g and 0.199 g, respectively, so that the seedling quality was better than that planted in rice tray (M1T0) with peat moss medium. The index of seedling quality A and B were used to evaluate the quality of purple coneflower seedlings in different media and trays. Seedlings planted in 60-cell plug tray with Taichung DAIS No 1. medium (M0T1) had best performance than that in rice tray (M1T0) with peat moss medium. However, the uptakes ability of P, K and Mg of seedling nutrient of purple coneflower in all trays with Taichung DAIS No 1. medium (M0T1) were better than that in the other trays with peat moss medium at 5 weeks after sowing.