細胞培養系統生產絞股藍皂苷之研究

Production of Gypenosides by Cell Culture System of Gynostemma pentaphyllum (Thunb.) Makino.

陳盈君(Ying-Chun Chen) ； 郭肇凱(Xhao-Kai Kuo) ； 張隆仁(Long-Zen Chang)

《臺中區農業改良場研究彙報》 96期 (2007/09) Pp. 1-9

https://doi.org/10.6956/BTDAIS.200709.0001

摘要

絞股藍為葫蘆科絞股藍屬植物，絞股藍皂苷（gypenosides）則是其重要活性成份之一。本研究完成建立絞股藍皂苷測定流程，並於1種本地採集材料測得絞股藍皂苷成份。在組織培養方面，葉片培植體培養於含有0.2～1.0 mg/l picloram之培養基皆有癒傷組織生成，0.2 mg/l picloram與低濃度BA（0～1 mg/l）組合之培養基同時有不定根生成。癒傷組織繼代培養於含有5 mg/l BA及0.2 mg/l picloram之基礎培養基，一個月後其鮮重增加約2.57倍，癒傷組織呈現黃白色、緊實之型態。液體培養至第7～10天後細胞量不再增加，細胞內容物充實，且細胞呈現團聚現象。

關鍵字

絞股藍 ； 七葉膽 ； 絞股藍皂苷 ； 癒傷組織 ； 細胞培養

並列摘要

Gynostemma pentaphyllum (Thunb.) Makino belongs to the Cucurbitaceae and its gypenosides closely resemble in ginsenoside. We established the GRg1 detection system and also found the similar component in a plant material native in Taiwan. Calli were induced from leaf explants on Murashige and Skoog (MS) basal medium plus 0.2-1.0 mg/l picloram. The first of these was pale yellow in color, compact in texture and watery in appearance. These calli could be maintained by subculturing every month with basal medium supplemented with 5 mg/l BA and 0.2 mg/l picloram. Liquid culture system was established by subculturing cells every 7-10 days.

並列關鍵字

Gynostemma pentaphyllum ； gypenoside ； callus ； cell culture