- 期刊
- OpenAccess
中藥草對蜜蜂球囊菌抑菌評估
Inhibitory effect of herbal extracts on the growth of Ascosphaera apis
摘要
蜜蜂球囊菌(Ascosphaera apis)導致蜜蜂幼蟲感染白堊病(chalk brood)，本研究利用4種中草藥萃取液對蜜蜂球囊菌病原菌抑制作用進行試驗，測試中草藥萃取液抑制菌絲生長情形，評估其防治白堊病之可行性，以發展天然資材與安全防治方法，比較萃取液單方(黃苓或丁香)與複方(黃苓+丁香)之抑菌效果，試驗結果顯示：以複方(7.5~10%)對菌絲抑菌率達100%，初步證實添加黃苓或丁香萃取液其抑菌作用最顯著。利用PDB添加複方濃度0~5%，測試最大抑菌濃度為3%時，即可完全抑制菌絲鮮重及乾重增加，且菌絲球( mycelia pellet)無法形成，顯示出黃苓與丁香複方抑制菌絲生長具協同作用。
並列摘要
Ascosphaera apis is a main fungal pathogen causing Chalk brood disease in honey bee larvae, seriously effect the beekeeping industry. To develop a safe controlling method, effect of four water herbal extracts on inhibitory of Ascosphaera apis and the growth of the fungi were evaluated. The results showed that the water extract of clove and skullcap have the strongest inhibiting effect on growth of Ascosphaera apis. Comparison of unilateral (skullcap or clove) and combinations (skullcap and cloves) herbal water extract, the later one showed higher antifungal inhibitory activity effect than the former one. The maximum inhibitory concentration of combinations was determined within 3%(v/v). Test confirmed that skullcap and cloves compound with synergistic inhibition of mycelial growth.
延伸閱讀
- 王全祿、呂俞慧、劉玉雯（2020）。中藥草精油抑菌活性之研究。中醫本草芳香療法期刊，4(2)，5-21。https://doi.org/10.6763/IJTCMA.202011_4(2).0001
- 王韻雯（2011）。臺灣蜂蜜之抗發炎及其活性特徵分析之研究〔碩士論文，國立虎尾科技大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0028-1407201116283600
- 蔡博宇（2011）。蜂子粉對人工蛹蟲草子實體之抗氧化活性分析〔碩士論文，國立虎尾科技大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0028-2601201102494800
- Kuo, Y. C., Lin, A. P., Lee, M. J., Chou, C. J., Fan, C. Y., & Tsai, W. J. (2003). Inhibitory Effects of Extracts from Vandellia Cordifolia on Tumor Cells Proliferation. 中醫藥雜誌, 14(3), 163-174. https://doi.org/10.6940/JCM.200309_14(3).03
- 林郁雯（2015）。Antioxidant activities and xanthine oxidase inhibitory effects of medicinal plants〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2015.02100