The anthracnose rot is one of the most important diseases of the strawberry (Fragaria × ananassa Duchesne), which causes serious damage either in the nurseries or in the production fields. In this study the isolate-propagated (Anthracnose-free) strawberry runner plants and ordinary ones were transplanted in the field and evaluated. The results showed that the isolate-propagated plants had significantly lower disease severity and mortality rate. Especially, the mortality rate of the isolate-propagation plants remained lower than 1%, while the ordinary ones around 30%. The adoption of the anthracnose-free runner plants could lower down the disease severity in the production field.