香芹酚(Carvacrol)在到手香〔Plectranthus amboinicus (Lour.) Spreng〕植物的根、莖及葉組織中的分佈及抗菌活性

Carvacrol Distribution and Anti-microbial Activities of Tissue Extracts of Plectranthus amboinicus (Lour.) Spreng

吳致怡(Chih-Yi Wu) ； 周志青(Jyh-Ching Chou)

《檢驗及品保雜誌》 4卷3期 (2015/08) Pp. 128-135

https://doi.org/10.6573/JTQA.201508_4(3).04

摘要

到手香學名Plectranthus amboinicus (Lour.) Spreng 為唇形花科多年生草本植物，也是一種民俗藥用植物，其活性成分中香芹酚(carvacrol)為一種單萜類酚化合物(monoterpenoid phenol)，具有消炎、鎮痛及抗發炎的作用。為了探討香芹酚在P. amboinicus植物中的根、莖及葉各部位的分布情形及其萃取物之抗生素活性，吾等以高效液相層析法(HPLC)做為分析儀器測量出根、莖及葉各部位香芹酚的含量，再比較乾燥過後的植物和新鮮植物的香芹酚的含量差異，結果顯示在根、莖及葉三部分的含量是以葉部含量最多，結果亦指出乾燥後的相對含量較新鮮組織高。P. amboinicus 的植物粗萃物對於革蘭氏陰性桿菌Escherichiacoli KRX（大腸桿菌）和革蘭氏陽性桿菌Arthrobacter ilicis D50 (冬青節桿菌)兩種菌株的生長抑制研究，結果顯示P. amboinicus 根、莖及葉的粗萃物對E. coli KRX 並無明顯抑制性，而對A. ilicis D50 則具有抑制性，尤其根部的粗萃液的抑菌效果更為顯著，此生長抑制特色與香芹酚者類似。進一步利用管柱色層分析所得到的根部萃取物進行A. ilicis D50 的抗菌試驗，亦出現明顯抑制圈，此指出抑菌成份可能與香芹酚有關。

關鍵字

到手香 ； 香芹酚 ； 高效液相層析 ； 藥用植物 ； 抗菌活性

並列摘要

Plectranthus amboinicus is a Lamiaceae perennial herbal plant and has been reported to carry some bioactive substances. Among the bioactive compounds studied, carvacrol (a monoterpenoid phenol) was of interest for its antiinflammatory and analgesic activities. This study tried to determine the tissue distribution of carvacrol in P. amboinicus as well as the biological activities of P. amboinicus tissue extracts toward an enterobacterium, Escherichia coli, and a soilborne bacterium, Arthrobacter ilicis and to evaluate the anti-microbial activity of carvacrol. Distributions of carvacrol in different tissues of P. amboinicus, including roots, stems and leaves, were determined by HPLC and confirmed with GC-MS. Analysis of P. amboinicus tissue extracts for its anti-microbial activities was performed against E. coli and A. ilicis by micro-plate bioassay technique. The results indicated that leaves contained carvacrol more than roots and stems did in dried or fresh samples. However, roots contained a more complex profile of phytochemicals. Analysis of P. amboinicus extracts for its anti-microbial activities indicated that P. amboinicus extracts did not inhibit the growth of E.coli, but inhibited A. ilicis. The root extracts carried highest anti-microbial activity than those of leaves and stems by a synergistic action of carvacrol with unknown polar compounds existing only in the root extract.

並列關鍵字

Antimicrobial activity ； carvacrol ； HPLC ； herbal medicine ； Plectranthus amboinicus