摘要
反轉醇(trans-3,5,4’-trihydroxystilbene；Resveratrol)，為存在植物中之一種多酚類化合物，是植物為了應變損傷如黴菌感染或暴露在紫外線照射下時，所產生的天然植物抗毒素(Phytoalexin)。常見於葡萄表皮、花生及紅酒中，對人類而言，反轉醇亦具有抗發炎及抗癌的功效。雖然，以往的研究顯示，反轉醇具有多種藥理活性，但截至目前為止，則甚少研究討論反轉醇在皮膚保養方面的功效。本研究的主要目的，在於探討反轉醇可能的美白功效。研究結果顯示，反轉醇可以有效抑制蕈菇酪胺酸酶之酵素活性，且由酵素動力學分析結果推測，反轉醇應為酪胺酸酶之非競爭性抑制劑。而反轉醇與麴酸相加後，其抑制細胞外酪胺酸酶活性能力具有加成作用。此外，反轉醇亦能明顯抑制老鼠B16F10黑色素瘤細胞內之酪胺酸酶活性，並降低細胞內之黑色素含量。後續研究將進一步設計含有反轉醇之有效配方，並將結果應用於皮膚美白保養品的開發。
並列摘要
Resveratrol (trans-3,5,4’-trihydroxystilbene) is a kind of polyphenolic compound existed in plants. It acts as plant antitoxin (phytoalexin) against mold infection or ultraviolet radiation-induced damage. Resveratrol is commonly found in grapes, peanuts, and red wine. Previous reports have shown that resveratrol express various pharmacological effects including anti-inflammation and anti-tumor. However, it is not clear whether resveratrol show potential skin care effects or not. The aim of this present study is to evaluate potential skin whitening properties of resveratrol. Our preliminary results indicated that resveratrol act as a non-competitive inhibitor for the mushroom tyrosinase. Besides, resveratrol and kojic acid act synergistically on the inhibition of such tyrosinase. Additionally, the intracellular tyrosinase activity and melanin content was decreased by resveratrol in murine B16F10 melanoma cells. Further study will design effective resveratrol-containing cosmetic formulations applied in research and development of skin whitening products.