化妝品常用二十八種精油之總酚含量測定、清除DPPH自由基能力、總抗氧化能力及主要精油化學成分分析之研究

Study of total phenolic contents, DPPH free-radical scavenging activity, trolox equivalent antioxidant capacity and their major chemical components analyses of twenty-eight commonly used essential oils

陳佳惠(Chia-Hui Chen) ； 黃秋菁(Chiu-Ching Huang) ； 陳雅筑(Ya-Ju Chen) ； 王曉芬(Hsiao-Fen Wang) ； 易光輝(Kung-Hway Yih)

《弘光學報》 69期 (2013/03) Pp. 1-11

https://doi.org/10.6615/HAR.201303.69.01

摘要

本研究分析來自化妝品常用之二十八種精油之總酚含量測定、清除DPPH自由基能力及總抗氧化能力，並進一步以氣相層析質譜儀（GC-MS）分析主要精油之化學組成。結果顯示總酚含量測定（TPC）、清除DPPH自由基能力（DFRS）、總抗氧化能力（TEAC），實驗證實TPC與TEAC、DFRS成正相關性，以冬季香薄荷（Savory, Satureja montana ）精油在實驗樣品中其抗氧化力最為顯著，當樣品濃度為1 mg/ml時，DFRS為73.74％，半數有效抑制濃度（EC_(50)）為294.51 μg/ml。以氣相層析質譜儀（Gas Chromatography-Mass Spectrophotometer, GC-MS）鑑定其主要化學成分，其主要成份含量有香芹酚（Carvacrol）59.71％與對-傘花烴（p -Cymene）22.01％、百里香酚 （Thymol）8.02％、α- 品烯（α-Terpinene）4.74％、β-石竹烯（β-Caryophyllene）2.72％、1R-α-蒎烯（1R-α-Pinene）1.56％，將其分別配置成濃度1mg/ml，進一步探討DFRS。其組成DFRS依序為：Thymol ＞ Savory ＞ Carvacrol ＞ β-Caryophyllene ＞ α-Terpinene ＞ p -Cymene 結果顯示酚化合物為主要抗氧化之成份。

關鍵字

總酚含量測定 ； 清除DPPH自由基能力 ； 總抗氧化能力 ； 半數有效抑制濃度 ； 冬季香薄荷精油

並列摘要

This study was to examine the in vitro antioxidant activities of twenty-eight commonly used essential oils and their major components by gas chromatography-mass spectrometry（GC-MS）. In the total phenolic contents（TPC）and the DPPH free radical scavenging ability（DFRS）and trolox equivalent antioxidant capacity（TEAC）, Savory essential oil is the best among these essential oils,the major chemical component was carvacrol. 1mg/ml of Savory essential oil was 73.74％ of the DPPH free radical scavenging ability and its EC_(50) is 294.51 μg/ml. At the concentration of 1mg/ml, Savory and five of its main components were designed to examine the DPPH free-radical scavenging activity. It is clear that the DPPH free radical scavenging activity is in the following order: thymol ＞ savory ＞ carvacrol ＞β-caryophyllene ＞ α-terpinene ＞ p -cymene , Thymol was the major component that attributed the high DPPH free-radical scavenging activity of savory essential oil.

並列關鍵字

Total Phenolic Content ； TPC ； DPPH Free Radical Scavenging Assay ； DFRS ； Trolox Equivalent Antioxidant Capacity ； TEAC ； EC_(50) ； Savory ； Satureja montana

參考文獻

Barja, G. (2004). Free radicals and aging. Trends Neurosci. 27, 595-600.

Daise, L. L., Alviano, D. S., Alviano, C. S. and Kolodziejczyk, P. P. (2008). Screening of chemical composition, antimicrobial and antioxidant activities of Artemisia essential oils. Phytochemistry . 69, 1732-1738.

Erkan, N., Ayranci, G. and Ayranci, E. (2008). Antioxidant activities of rosemary (Rosmarinus Officinalis L.) extract, blackseed (Nigella sativa L.) essential oil, carnosic acid, rosmarinic acid and sesamol. Food Chem . 110, 76-82.

Gholivand, M. B., Mehdi, R. N., Batooli, H. and Ebrahimabadi, A. H. (2010). Chemical composition and antioxidant activities of the essential oil and methanol extracts of Psammogeton canescens. Food Chem. Toxicol. 48, 24-28.

Hussain, A. I., Anwar, F., Sherazi, S. T. H. and Przybylski, R. (2008). Chemical composition, antioxidant and antimicrobial activities of basil (Ocimum basilicum ) essential oils depends on seasonal variations. Food Chem. 108, 986-995.