台灣嘉寶果萃取及分離物之化妝品活性評估

Evaluation of the cosmetic activity of extracts and fractions of Taiwan’s Myrciaria cauliflora

李姿芳(Tz-Fang Li) ； 羅思璇(Szu-Hsuan Lo) ； 徐照程(Jaw-Cherng Hsu)

《弘光學報》 69期 (2013/03) Pp. 82-95

https://doi.org/10.6615/HAR.201303.69.07

摘要

本研究選用在地培植之嘉寶果品種之果皮與果肉，利用超音波輔助溶劑萃取法，比較不同溶劑的萃取效能，並以總酚含量、DPPH 自由基清除率、清除ABTS^(.+)自由基清除能力、酪胺酸酶抑制能力方法，評估萃取物及分離物之生物活性。結果顯示，超聲波萃取輔助 95 % 乙醇∕水 (1:1) 嘉寶果萃取物 (JAW) 具有較高的總酚含量與DPPH 自由基清除能力，為適合嘉寶果多酚成份的萃取溶劑。其分佈萃取之乙酸乙酯分離物 ( JAWE)，具有極高量的酚類成份，為強抗氧化力成份石榴花萃取物的 2 倍。優異的 DPPH 自由基清除能力與 ABTS^(.+)自由基清除率，分別為常見抗氧化劑 BHT 的 3.1 倍 與 Trolox 的 1.9 倍。並且有強大的酪胺酸酶抑制作用，更勝出市售美白產品添加成份熊果素的 7.8 倍。綜合多種生物活性試驗評估結果，嘉寶果萃取及分離物有抗氧化、美白之功效，具有開發成為化妝品活性成份之潛力。

關鍵字

台灣嘉寶果 ； 抗氧化力 ； 萃取法 ； 分離物 ； 酪胺酸酶抑制劑 ； 化妝品

並列摘要

The skin and flesh of Taiwan-grown Myrciaria cauliflora (Jaboticaba) was extracted by ultrasoundassisted method. We have assessed the antioxidant and anti-tyrosinase activity of various solvent extracts and fractions of Jaboticaba by total phenolic content, DPPH radical scavenging and ABTS^(.+) radical scavenging assay methods. The ultrasonic-assisted extraction of Jaboticaba with 95% ethanol / water (1:1) (JAW) had a higher total phenolic content and DPPH radical scavenging capacity than other conditions. Further partitioning with ethyl acetate fraction (JAWE) had highest phenolic compounds contents, even much better than the pomegranate flower extract, which was famous with antioxidant ability. Excellent DPPH radical scavenging ABTS^(.+) radical scavenging ability were 3.1 times and 1.9 times than common antioxidant BHT and Trolox. JAWE fraction was also a powerful tyrosinase inhibitor, which better than commercial cosmetic whitening ingredient such as arbutin (7.8 times). Jaboticaba extracts and fractions possess antioxidant activity and whitening effect, with the development potential as novel cosmetic active ingredients.

並列關鍵字

Taiwan Myrciaria cauliflora ； antioxidant activity ； extraction method ； fraction ； tyrosinase inhibitor ； cosmetic

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