Application of Hydrochloric Acid Decalcification Solution in Preparation and Section of Intervertebral Disc in Mice

Xiaoling Chen ； Lek Hang Cheang ； Jiaqing Zhang

《International Core Journal of Engineering》 8卷6期 (2022/06) Pp. 502-508

https://doi.org/10.6919/ICJE.202206_8(6).0066

摘要

The pathological section of bone tissue can observe the whole picture of bone tissue, which is very important for determining the clinical prognosis. However, the rich calcium salt in bone tissue makes it difficult to make a complete film. At present, the 10%EDTA decalcification method is a generally accepted method that takes a long time but is not suitable for clinical diagnosis. Acid decalcification is increasingly becoming the first choice for the treatment of bone specimens. The intervertebral discs of mice were collected and embedded in paraffin sections after two different decalcification methods. H&E staining, Safranine O-Fast Green staining, Picrosirius Red-Alcian Blue staining and immunohistochemistry were performed respectively. The results showed that the two methods could clearly show each layer of tissue and the shape of intervertebral disc was complete. The Aggrecan positive protein could be expressed in all of them. In this experiment, 8% hydrochloric acid decalcification solution was applied to the preparation and staining process of mice intervertebral disc, in order to popularize and apply in the treatment of clinical bone lesions.

關鍵字

Intervertebral Disc ； Decalcified Solution ； H&E Staining ； Safranine O-Fast Green Staining ； Picrosirius Red-Alcian Blue Staining

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