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接種乳酸桿菌對水稻穀粒青貯品質之影響

Effect of Lactobacillus spp. inoculation on the silage quality of rice (Oryza sativa L.) grain

摘要


本試驗以臺南區農業改良場嘉義分場提供之臺中秈17號(TCS17)水稻品種之穀粒為材料,探討添加畜產試驗所恆春分所提供之不同乳酸桿菌(Lactobacillus spp.)對水稻榖粒青貯料之營養成分與發酵品質的影響,以供草食動物食用芻料之參考。將試驗水稻榖粒青貯調製30及60天後,分析其粗蛋白質、水溶性碳水化合物、中洗纖維、酸洗纖維、澱粉與磷、鉀及鎂等。青貯發酵品質之分析項目包括酸鹼值(Ph)與乳酸、乙酸、丁酸等有機酸含量,並計算費氏品質評分(Flieg's scores)。結果顯示,接種乳酸桿菌在進行青貯30天後的水稻榖粒青貯料之pH值3.8顯著(P < 0.05)低於只噴水不添加乳酸菌之對照組之pH 4.4,接種處理亦顯著提高乳酸含量至1.1%,並抑制丁酸之產生,故所有接種乳酸菌處理之Flieg's scores評定為優良之80分以上,其中ST15乳酸菌品系的處理可達89分,大幅高於對照組之23分;青貯60天的處理亦有相同的結果。因此,為提升水稻穀粒青貯料品質,建議可接種乳酸桿菌ST15品系後青貯,可獲得優良的水稻榖粒青貯料。

並列摘要


In order to improve the problem of sale public stock rice grains as feeds source which had been stored for a long time and the quality of grains became bad. The grains of Taichung Sen 17 (TCS17) were ensiled for 30 and 60 days used to investigate the influence of lactic acid bacteria (Lactobacillus spp.) inoculated for rice grain ensiling to provide information for ruminant farmers. The nutrition contents analysis of rice grain silage were determined 30 and 60 days after ensiling, respectively. Analysis included crude protein (CP), water soluble carbohydrate (WSC), neutral detergent fiber (NDF), acid detergent fiber (ADF), starch and phosphorous, potassium and magnesium of mineral content. The pH value and the volatile fatty acid i.e. lactic acid, acetic acid and butyric acid were determined. The results showed that the pH 3.8 of inoculated with Lactobacillus spp. was lower than pH 4.4 of control with water (CKW) treatment. The lactic acid reached to 1.1% and inhibited the butyric acid to produce when Lactobacillus spp. was inoculate to grain before ensiling. The pH value and Flieg's scores of rice grain silage were about 3.80 and 80 points 30 days after ensiling, respectively. The Flieg's scores of grain silage inoculated with line ST15 of Lactobacillus spp. was 89 points higher than that of CKW treatment with 23 points. Results of ensiling for 60 days of all treatments were the same as those of ensiling for 30 days. The results suggested that inoculation with Lactobacillus spp. on ensiling could effectively enhance the quality of rice grains silage.

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