錫蘭橄欖葉子萃取物抑制斑馬魚黑色素活性之研究

Melanogenesis Inhibition Activity in Zebrafish (Danio rerio) of Elaeocarpus serratus Leaf Extract

黃祥哲(Xing-Zhe Huang)

指導教授 ： 張惠婷

國立臺灣大學/生物資源暨農學院/森林環境暨資源學研究所/碩士(2018年)

https://doi.org/10.6342/NTU201800008

摘要

本研究評估錫蘭橄欖 (Elaeocarpus serratus) 葉子乙醇萃取物對酪胺酸酶的抑制效果，以及對斑馬魚黑色素生成的影響。顯示錫蘭橄欖葉子萃取物各分離部中，以乙酸乙酯可溶部具有最佳的抑制酪胺酸酶活性，以及減少斑馬魚 (Danio rerio) 黑色素生成的能力。乙酸乙酯可溶部各次分離部中以 E7 次分離部具有最好的抑制酪胺酸酶活性，同時可有效降低斑馬魚黑色素的生成。酵素動力學分析中，無論以酪胺酸或多巴為基質時，E7 次分離部均為競爭型抑制。
分析 E7 次分離部化合物抑制酪胺酸酶的活性，以 mearnsetin 具有最佳抑制效果，由酵素動力學分析得知，無論使用酪胺酸或多巴為基質時，gallic acid、mearnsetin 與 myricitrin 皆為競爭型抑制劑，而 myricetin 為混合型抑制劑。在斑馬魚黑色素的活性評估，化合物抑制黑色素生成的能力依序為 mearnsetin > myricitrin > myricetin > gallic acid。綜合研究結果，顯示錫蘭橄欖葉子萃取物及其化合物抑制酪胺酸酶活性良好，並可抑制斑馬魚的黑色素，具有開發成為美白劑的潛力。

關鍵字

抗酪胺酸?活性 ； 錫蘭橄欖 ； 黑色素 ； 斑馬魚

並列摘要

The purpose of this study is to evaluate the antityrosinase activicity and melanogenesis inhibition activity in zebrafish of leaf ethanolic extract from Elaeocarpus serratus. Result shows that the ethyl acetate soluble fraction exhibited the highest antityrosinase activicity and melanogenesis inhibition activity in zebrafish. Among the subfractions of ethyl acetate soluble fraction, subfraction E7 possessed the best antityrosinase activity, and the inhibition mechanism were both competition type whether using L-tyrosine or L-Dopa as the substrate.
Results from the antityrosinase assay demonstrate that mearnsetin possessed the highest inhibition activity amony the compounds of subfraction E7. In the enzyme kinetic analyses, gallic acid, mearnsetin, and myricitrin were the competitive inhibitors, and myricetin was the mix-type inhibitor against tyrosinase whether using L-tyrosine or L-Dopa as the substrate. The melanogenesis inhibition activity in zebrafish of compounds examined was in a decreasing order of mearnsetin > myricetin > myricitrin > gallic acid. According to the results, E. serratus leaf extract and its compounds have good effects on the antityrosinase activity and melanogenesis inhibition activity in zebrafish, and are of the potential to be used as the skin whitening agent.

並列關鍵字

antityrosinase activity ； Elaeocarpus serratus ； melanin ； zebrafish (Danio rerio)

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