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  • 學位論文

介電質微米球透鏡之超解析成像性質研究

Super-Resolution Imaging through Dielectric Microspheres

指導教授 : 陳智泓
共同指導教授 : 張之威(Chih-Wei Chang)

摘要


可見光波段的超解析(super-resolution)影像一直是科學家努力在研究的課題。許多生物及醫學上的樣本,需要可見光波段才能觀察到其一般行為。但許多樣本的表現和特徵的觀察同時又需要超越可見光繞射極限的解析度。在過往的十幾年內,科學家不斷嘗試利用各種精確控制的奈米結構來放大及傳遞消逝波(Evanescent wave) 到遠(Far-field),或是利用特殊的掃描方式來重組影像以達到超解析的需求。雖然這些方法已經在實驗上有很顯著的成效,但其結構的複雜,製程上的高難度以及耗時的擷取影像程序卻成為其在應用上的致命傷。近年來,微米球白光超解析現象的發現讓人們得以用最簡單製程和直接觀察的方式以可見光的媒介來取得超解析影像,此發現無疑是光學領域的大突破。在我們的研究中,嘗試著藉由一些創意上的改良,使的微米球成像技術可以更加穩定,甚至可以自由地將這樣的超解析鏡頭轉移到各種我們想觀察的樣本上。另外我們也用電腦模擬來探討微米球的成像性質並與實驗比對,希望能更進一步了解其成像的基本機制。

並列摘要


It has been a long time since scientists put their efforts on obtaining super-resolution images for biological or medical applications. In the past few decades, scientists tried to magnify and transmit evanescent waves to far field by fabricating novel nanostructures or utilizing special scanning techniques to recombine images to achieve the super-resolution condition. Although significant advancements have been demonstrated, the sophisticated structures, difficulty in manufacturing, and time consuming process in obtaining images have hindered the widespread applications. Recently, the discovery of super-resolution under white-light illumination via microsphere lenses have helped us to achieve super-resolution images by direct observation via visible light without complex fabrication processes, making a great breakthrough in the field of optical imaging. In the thesis I try to improve the stability of the image quality by non-volatile polymer semi-immersion mechanism. Furthermore, I succeed in transferring microsphere superlenses to various kinds of samples of interests. Besides I also employ computer simulations to investigate the imaging characteristics of microspheres and try to have better understanding of the mechanism on microsphere-based super-resolution.

參考文獻


Chapter 1
1. Coene, W., et al., Phase retrieval through focus variation for ultra-resolution in field-emission transmission electron microscopy. Physical Review Letters, 1992. 69(26): p. 3743-3746.
2. Reimer, L., Scanning Electron Microscopy. (Berlin: Springer-Verlag), 2000.
3. Bining, G. and H. Rohrer, Scanning tunneling microscopy. IBM Journal of Research and Development, 1986. 30: p. 4.
5. Betzig, E. and J.K. Trautman, NEAR-FIELD OPTICS - MICROSCOPY, SPECTROSCOPY, AND SURFACE MODIFICATION BEYOND THE DIFFRACTION LIMIT. Science, 1992. 257(5067): p. 189-195.

被引用紀錄


劉運儒(2014)。以共軛焦顯微鏡觀察介電質微米球之光學超解析特性之研究與應用〔碩士論文,國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2014.10805

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