長途客運司機的氧化性傷害

Evaluation of Urinary 8-Hydroxydeoxyguanosine in Long Distance Bus Drivers

韓岳穎(Yueh-Ying Han)

指導教授 ： 林嘉明 ； 宋鴻樟

國立臺灣大學/公共衛生學院/環境衛生研究所/碩士(2004年)

https://doi.org/10.6342/NTU.2004.00503

摘要

空氣污染物中含有多種活性氧化物種（Reactive Oxidative Species, ROS），暴露在空氣污染中會增加細胞氧化性壓力，同時會造成DNA的氧化性傷害。運輸業的駕駛人員工作時間長，且長期暴露於交通廢氣污染的環境中。一般職業流行病學研究多針對工作壓力或健康體能評估運輸業駕駛人員的工作傷害。本研究的研究對象是以台灣地區120位男性長途客運司機為暴露組，58位男性站務人員為對照組，進行尿液中8-烴基去氧鳥糞嘌呤核苷（8-hydroxydeoxyguanosine, 8-OHdG）濃度測定，探討職業暴露以及生活型態與DNA氧化性傷害的關係。實驗結果發現客運司機尿液中的8-OHdG平均濃度高於站務人員的（9.5 ± 5.7 vs. 7.3 ± 5.4 µg/g creatinine, p＝0.015），同時每週工作時數較長的司機其尿液中有較高濃度的8-OHdG；校正其他影響因子後，司機的8-OHdG濃度高於7.3 µg/g creatinine之勝算比為站務人員的7.8倍（95% CI：2.1-29.5），而抽菸者有較高8-OHdG濃度的勝算比是不抽菸者的20.3倍（95% CI：6.4-64.0），同時8-OHdG的濃度會隨著尿液中cotinine濃度的上升而明顯地增加（p＜0.0001）。多變項線性迴歸分析結果發現，尿液中的8-OHdG濃度和客運駕駛、抽菸、飲用提神性飲料、以及尿液中的cotinine濃度有明顯相關性，因此，尿液中8-OHdG的濃度可反應出司機暴露於工作環境和抽菸所造成氧化性傷害。

關鍵字

8-烴基去氧鳥糞嘌呤核苷氧化性傷害 ； 長途客運司機

並列摘要

Cellular oxidative stress and damage to DNA may involve the increased risk of diseases associated with air pollution, which contains a number of reactive oxygen species （ROS）. Professional drivers work in the environment with high physical stress and long-term exposure to traffic exhaust. To investigate the role of environmental and occupational factors associated with oxidative DNA damage, the levels of urinary 8-hydroxydeoxyguanosine （8-OHdG）, a biomarker of DNA oxidative damage, were determined for 120 long-distance bus drivers as exposure group and 58 office clerks as non-exposure group for comparison. The results indicated that the average level of urinary 8-OHdG in the drivers was higher than that in the clerks （9.5 ± 5.7 vs. 7.3 ± 5.4 µg/g creatinine; p＝0.015）, and the urinary 8-OHdG levels in bus drivers increased significantly as the weekly work hours increased. After adjusting for demographic characteristics and lifestyles in multivariate logistic regression, drivers were at higher risk than the clerks（odds ratio (OR)＝7.8, 95% CI：2.1-29.5）to have the 8-OHdG levels above 7.3 µg/g creatinine; and so were smokers than non-smokers（OR＝20.3, 95% CI：6.4-64.0）. A strong dose-response relationship revealed when the urinary 8-OHdG levels were depicted with the elevation of urinary cotinine（p＜0.0001）. In the multiple regression analysis, the results showed that bus driver group, smoking, consumption of energy supplement drinks, and increasing urinary cotinine level were significant predictors of 8-OHdG in the urine. Our study suggests that urinary 8-OHdG detection can be used to assess the oxidative damage of smoking and occupational exposure by comparing 8-OHdG data between control group and exposure group in epidemiology studies.

並列關鍵字

8-hydroxydeoxyguanosine ； oxidative damage ； bus drivers

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