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  • 學位論文

調控銅奈米團簇聚集體大小並應用於紙質平臺偵測硫化氫

Size-tunable copper nanocluster aggregates and their application in hydrogen sulfide sensing on paper-based devices

指導教授 : 張煥宗
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摘要


本篇論文主要在開發一步合成法,利用加入不同濃度的聚苯乙烯磺酸鈉(polystyrene sulfonate)控制青黴胺(penicillamine)-銅奈米團簇聚集大小。相較於沒有加入聚苯乙烯磺酸鈉的銅奈米團簇,經由聚電解質調控聚集體大小的銅奈米團簇具有很好的分散性、光穩定性以及對於硫化氫有很好的選擇性與靈敏性,對於硫化氫的偵測極限為650 nM,因此可應用於偵測溫泉水樣品中之硫化氫。此外,更將此發光銅奈米團簇與可攜式紙質分析裝置(microfluidic paper-based analytical device)結合,直接使用肉眼判定溫泉水中硫化氫之有無以及微量盤式螢光儀定量硫化氫濃度。有別於傳統的硫化氫檢測方法只能於溶液相進行檢測,我們利用加熱溫泉水後會釋放出硫化氫氣體,可與紙質平臺上的銅奈米團簇反應後使其螢光消光,可直接使用肉眼進行快速判定,此方法亦可避免樣品中一些金屬離子的干擾;而使用微量盤式螢光儀直接定量硫化氫濃度於紙質裝置上,此方法相較於傳統公告方法只需非常小的樣品體積(5 μL)以及較短的分析時間(30分鐘),對於硫化氫的偵測極限為1 μM。由於此發光銅奈米團簇/紙質裝置具有製備簡單、成本低、可拋棄、快速偵測且精準等優點,因此具有很大的發展潛力於監控氣相與液相硫化氫之含量。

並列摘要


An one-pot approach has been developed to control the size of penicillamine-copper nanocluster (PA-Cu NC) aggregates through the addition of different concentrations of polystyrene sulfonate (PSS). The PSS-PA-Cu NC aggregates are photoluminescent and have excellent water dispersity, better photostability under UV irradiation, and remarkable sensitivity towards H2S (limit of detection: 650 nM) compared to PA-Cu NC aggregates without PSS dispersion. The practicability of this probe was verified by determination of the H2S concentration in hot spring-water samples. The PSS-PA-Cu NCs were integrated into a portable microfluidic paper-based analytical device (μPAD) for the on/off determination of H2S in hot spring-water samples. This platform targets released H2S gas to avoid interference from other ions in water samples in contrast with conventional H2S detection methods which can only determine H2S directly from solution. The PSS-PA-Cu NC/μPADs use ultra-low sample volumes (5 μL) and feature shorter analysis times (~30 min) compared to conventional solution-based methods. The quantitative results not only can be seen by the naked-eye, as well as facilely recorded and transmitted using a smartphone but also can determined by microplate reader. The LOD of the PSS-PA-Cu NC/μPAD device was 1 μM. In conclusion the PSS-PA-Cu NC/μPADs have great potential to monitor H2S levels in gaseous and liquid samples.

參考文獻


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