肝硬化相關細胞激素對B型肝炎誘導肝細胞癌之預測：蛋白質微陣列分析

Identification of Cytokines Involved in HBV-Related Cirrhosis for Hepatocellular Carcinoma Prediction: A Protein Microarray Analysis

劉家綺(Chia-Chi Liu)

指導教授 ： 于明暉

共同指導教授 ： 莊曜宇

國立臺灣大學/公共衛生學院/流行病學研究所/碩士(2009年)

https://doi.org/10.6342/NTU.2009.01091

摘要

無資料

關鍵字

細胞激素 ； B型肝炎 ； 肝硬化 ； 肝癌 ； 微陣列晶片

並列摘要

Background Early identification of high risk population for liver cirrhosis may be beneficial to hepatitis B virus (HBV) carriers and their prognosis on hepatocellular carcinoma (HCC). We aimed to identify differently expressed cytokines in plasma between clinical liver cirrhosis patients and asymptomatic HBV carriers as biomarkers for HCC prediction. Methods A total of 174 cytokines were assessed in plasma from 16 HBsAg carriers at different stages of liver cirrhosis using cytokine antibody microarrays. Ten promising cytokines were later evaluated in a case-control study including 49 HCC cases and 50 matched controls nested within a longitudinal cohort study of HBsAg carriers by using quantitative antibody arrays. Blood samples from each study subject were collected up to 16 years before diagnosis of HCC. Results Forty-five cytokines were differentially expressed among asymptomatic carriers, Child-Pugh stage A patients and Child-Pugh stage B/C patients (parametric P-value range: 0.0006-0.0487, empirical P-value range: 0.0003-0.0556, false-discovery rate q-value range=0.0713-0.1244). Evaluation of a subset of 10 cytokines, including ICAM-2, MCP-1, EGF, CXCL-16, IGFBP-2, sTNFRII, TIMP4, Fas, RANTES, and TIMP-1, by a nested case-control study showed that only plasma ICAM-2 levels were significantly different between HCC cases and controls at baseline (P-value for Wilcoxon sum rank test = 0.0413). Plasma ICAM-2 levels exhibited an accuracy (AUC=0.6208) comparable to HBV viral load (AUC=0.6022) and superior to serum ALT (AUC=0.5965) for prediction of HCC. Conclusion Results of this study suggested that ICAM-2 may serve as a promising noninvasive biomarker for prediction of HCC development. Key Words Cytokines, Hepatitis B, liver cirrhosis, hepatocellular carcinoma, microarrays

並列關鍵字

Cytokines ； Hepatitis B ； liver cirrhosis ； hepatocellular carcinoma ； microarrays

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