綠竹筍殼蔗糖磷酯合成酶之生化學研究

Biochemical Studies on Sucrose Phosphate Synthase from Shell of Bamboo (Bambusa oldhami)

盧厚任(Hou-Ren Lu)

指導教授 ： 李平篤

國立臺灣大學/生命科學院/微生物與生化學研究所/碩士(2007年)

https://doi.org/10.6342/NTU.2007.00836

摘要

蔗糖磷酯合成酶 (sucrose phosphate synthase, SPS, EC 2.4.1.14) 是植物蔗糖生合成的重要調控酵素，本論文以綠竹筍之筍殼為材料，進行 SPS 之生化學研究。粗抽液經陰離子交換層析 (DEAE-Sepharose CL-6B chromatography) 後，發現有兩種 SPS 活性分劃，分別經親和層析 (

關鍵字

蔗糖磷酯合成酶異構酶

並列摘要

Sucrose phosphate synthase (SPS, EC 2.4.1.14) is an important regulatory enzyme in sucrose-biosynthetic pathway. In this study, SPS was isolated from bamboo shells and two distinguished isofroms were identified using DEAE-Spharose CL-6B chromatography. The two isoform of SPS were then further purified by 24% PEG precipitation,

並列關鍵字

sps ； sucrose phosphate synthase ； isozyme

參考文獻

Chen WL, Huang DJ, Liu PH, Wang HL, Su JC, Lee PD 2001 Purification and characterization of sucrose phosphate synthase from sweet potato tuberous root. Bot. Bull. Acad. Sin. 42: 123-129

Amir, J., and Preiss, J. 1982. Kinetic Characterization of Spinach Leaf Sucrose-Phosphate Synthase. Plant Physiol. 69(5): 1027-1030.

Babb, V.M., and Haigler, C.H. 2001. Sucrose phosphate synthase activity rises in correlation with high-rate cellulose synthesis in three heterotrophic systems. Plant Physiol. 127(3): 1234-42.

Bruneau, J.M., Worrell, A.C., Cambou, B., Lando, D., and Voelker, T.A. 1991. Sucrose Phosphate Synthase, a Key Enzyme for Sucrose Biosynthesis in Plants: Protein Purification from Corn Leaves and Immunological Detection. Plant Physiol. 96(2): 473-478.

Chen, S., Hajirezaei, M., and Bornke, F. 2005. Differential expression of sucrose-phosphate synthase isoenzymes in tobacco reflects their functional specialization during dark-governed starch mobilization in source leaves. Plant Physiol. 139(3): 1163-74.