本實驗研究目的在於探討飼料中添加不同濃度樟芝菌絲體培養基對南美白蝦非特異性免疫能力的影響。在飼料中添加0(Ctrl)、0.75%(A)、1.25%(B)、2.5%(C)、5%(D)之樟芝菌絲體培養基連續投餵15天,分別於0、1、3、6、9、15天抽血檢測其原酚氧化酵素(Prophenoloxidase, proPO)活性、超氧陰離子(O2-)生產率、吞噬作用活性(Phagocytic activity)、溶菌酶活性(Lysozyme activity)等非特異性免疫指標,進一步評估樟芝菌絲體培養基是否能提升南美白蝦(Litopenaeus vannamei)的免疫能力。結果顯示A、B、C組皆能有效提升血檢測其原酚氧化酵素(proPO)活性、吞噬作用活性、溶菌酶活性,但提昇效果沒有隨著添加樟芝菌絲體培養基濃度增加而上升,其中以1.25%(B)效果最好。A、B組在第六天可有效提升超氧陰離子(O2-)生產率,但D組別在第十五天有抑制超氧陰離子(O2-)生成的現象。 綜合實驗結果發現,添加樟芝菌絲體培養基於南美白蝦飼料中,可以有效提升南美白蝦非特異性免疫能力,若要使用樟芝菌絲體培養基作為免疫激活物投餵,建議使用劑量為0.75~1.25%
The study aims to investigate the effects of Antrodia camphorate medium on non-specific immune response of white shrimp (Litopeneaus vannamei). Antrodia camphorate medium have been added to shrimp diets with 0g/kg (Ctrl), 7.5g/kg (A), 12.5g/kg (B), 25g/kg (C) and 50g/kg (D) respectively. The immune responses, prophenoloxidase activity (proPO), superoxide anion production (O2-), phagocytic activity and lysozyme activity were examined after feeding for 0, 1, 3, 6, 9, 15 days. The results indicated that prophenoloxidase activity, phagocytic activity and lysozyme activity all significantly increased (p<0.05) when fed the shrimp were fed A、B、C diets. The superoxide anion production (O2-) were significantly higher (p<0.05) when fed the shrimp were fed A、B、C diets on sixth day. The present study showed that diet supplemented with Antrodia camphorate medium could elevate non-specific immune index of white shrimp. Thus, this study recommends that using 0.75~1.25 g/kg Antrodia camphorate medium as immunostimulants elevates non-specific immune of white shrimp.