疏花魚藤之化學成分研究

Studies on the Chemical Constituents of Derris laxiflora Benth.

邱錫臨(Hsi-Lin Chiu)

指導教授 ： 郭悅雄

國立臺灣大學/理學院/化學研究所/博士(2007年)

https://doi.org/10.6342/NTU.2007.00871

摘要

疏花魚藤（Derris laxiflora Benth.）為荳科植物，並且是台灣特有種，此植物生長在臺灣低海拔1000公尺以下，此植物的根與莖曾經被使用作為農用殺蟲劑，而台灣原住民也常取其根部的汁液毒魚，作為捕魚的一種方法。
本研究將疏花魚藤，以甲醇浸泡得到之萃取物，運用矽膠薄層分析、矽膠管柱層析、高效率液相層析儀及再結晶等方法進行分離純化。單離之純化合物經測定紅外線光譜、氫核磁共振光譜、碳核磁共振光譜與質譜後，經推測若為已知化合物者，與文獻值比對確定其結構。若為新化合物，依需要進行二維核磁共振光譜實驗，配合化學反應等方法確定其結構。
本實驗鑑定得到共143個化合物，分別為：
一、芳香族類（Aromatic compounds）化合物13個
二、葉綠素（Chlorophyll）化合物5個
三、類黃酮類（Flavonoids）化合物25個
四、異黃酮類（Isoflavonoids）化合物13個
五、木酚素（Lignans）化合物12個
六、紫檀烷類（Pterocarpans）化合物10個
七、固醇類（Steroids）化合物21個
八、三萜類（Triterpenoids）化合物32個
九、其他類（Other compounds）化合物12個
共計有15個新化合物和7個首次自天然界分離得到之化合物。

關鍵字

疏花魚藤 ； 魚藤屬 ； 類黃酮 ； 三萜類 ； 紫檀烷 ； 固醇類

並列摘要

Derris laxiflora Benth., a member of the Leguminosae, endemic in Taiwan, distributed at altitudes of under 1000 meters. It's roots and the stems were once used as the agricultural pesticide. Taiwan aboriginals also took root's juice to poison fish. The components can be used to poison fish, but innocuous to mammalian.
The methanol extract from D. laxiflora was concentrated to give a residue which was partitioned between ethyl acetate and H2O. The ethyl acetate layer was separated by column chromatography on silica gel, preparative thin layer chromatography, flash column chromatography, and high performance liquid chromatography (HPLC) to give one hundred and forty three compounds, including 13 aromatic compounds, 5 chlorophylls, 25 flavonoids, 13 isoflavonoids, 12 lignans, 10 pterocarpans, 21 steroids, 32 triterpenoids, and 12 other type compounds. Known compounds were identified by IR, NMR, and Mass spectral analysis and comparison with those of authentic samples or data reported in literatures. Structures of new compounds were further elucidated by 2D NMR spectral and chemical methods. Among these isolated compounds, there are fifteen new compounds, and seven of them were isolated for the first time from natural sources.

並列關鍵字

Derris laxiflora ； Derris ； flavonoid ； triterpenoid ； pterocarpan ； steroid

參考文獻

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