建立黑豆植化素指紋圖譜分析平台

Developing a Platform for Analyzing Phytochemical Fingerprinting in Black Soybean (Glycine max)

陳宏權(Hong-Quan Chen)

指導教授 ： 陳宏彰

國立臺灣大學/生物資源暨農學院/食品科技研究所/碩士(2017年)

https://doi.org/10.6342/NTU201703857

摘要

黑豆(Glycine max)富含植化素，植化素可作為黑豆之化學特徵，用以鑑別不同品種之黑豆。本研究建立黑豆植化素的分析平台，探討黑豆中植化素組成資訊。使用超高效能液相層析串聯質譜儀(UHPLC-DAD-ESI-MS)分別進行花青素及酚酸的定性分析，共鑑定出黑豆中6種花青素及10種酚酸。使用高效能液相層析串聯光二極體陣列檢測器(HPLC-DAD)定量分析異黃酮、花青素及酚酸，結果顯示，黑豆中異黃酮以丙二醯型及醣苷型為主，而乙醯型及苷元型含量稀少，其中又以genistein苷元結構之總含量最高；花青素定量分析發現cyanidin-3-O-hexoside含量最多，佔了超過60%的花青素總含量；酚酸有游離態與結合態兩種型態，游離態酚酸中有高比例的p-coumaric acid，結合態酚酸主要為protocatechuic acid，其次為syringic acid及vanillic acid，總酚酸則是以p-coumaric acid含量最高。以UHPLC-ESI-MS分析黑豆指紋圖譜，搭配主成分分析可將不同品種之黑豆分群，並區分出不同產地。本研究建立之分析平台能夠測定黑豆中的植化素，利用其化學資訊可以鑑別不同品種之黑豆。

關鍵字

黑豆 ； 植化素 ； 高效能液相層析串聯質譜儀 ； 指紋圖譜

並列摘要

Black soybean (Glycine max) is rich in phytochemicals. Phytochemicals could be used to diffentiate black soybean according to species. The characteristic chemical composition could be used to evaluate its quality. In this research, a method for the analysis of phytochemicals in black soybean was developed. Qualititative analysis of anthocyanin and phenolic acid was done by UHPLC-DAD-ESI-MS. A total of six anthocyanins and ten phenolic acids were identified in black soybean. Quantitative analysis of isoflavone, anthocyanin and pheolic acid was done by HPLC-DAD. Results showed that malonylglucoside and glucoside isoflavone were the major form in black soybean with less amount of acetylglucoside and aglycone isoflavone. Among the three aglycone structures, genistein had the highest amount. The analysis of anthocyanin indicated that cyanidin-3-O-hexoside was the main compound in black soybean, containing over 60% of total anthocyanin. Phenolic acid could be divided into free form and bound form. In this study, p-coumaric acid was the main compound among the free form phenolic acids and protocatechuic acid was the main compound among the bound form phenolic acids. The content of p-coumaric acid was the highest among all phenolic acids. Fingerprinting of black soybean was done by UHPLC-ESI-MS. Data from the fingerprinting was extracted and analysed by principal component analysis to group the black soybeans according to species. Differentiation of the origin of black soybean was also achieved. In conclusion, this study developed a method for the analysis of phytochemicals in black soybean and the chemical information could differentiate black soybean according to species.

並列關鍵字

Black soybean ； phytochemicals ； HPLC-DAD-ESI-MS ； fingerprinting

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