氧化銦鎵鋅薄膜電晶體結合電化學系統應用於生物化學反應之分析

這篇論文介紹以氧化銦鎵鋅薄膜電晶體組成之生物感測器的不同型態及用途，此研究分為兩部分：第一部分，使用薄膜電晶體結合微流道作為感測的平台，以菸鹼醯胺腺嘌呤二核苷酸(還原形式)，蘋果酸脫氫酶(MDH)和草醯乙酸(OAA)之間的化學反應動力學為研究標的物。首先，將不同濃度的菸鹼醯胺腺嘌呤二核苷酸的氧化形式(NAD+)與還原形式(NADH)溶液分別注入微流體通道，實時地監測電流訊號。並通過擴散模型的擬合，得出汲極電流隨時間變化的函數。接著，測量不同濃度倍數的NADH，OAA和MDH混合溶液。透過生化公式以及數據結果可提供解離常數等信息。值得注意的是，導出的平衡常數為（8.06 ± 0.61）× 104，與其他團隊的研究結果相近。第二部分，將薄膜電晶體作為負回饋元件，並結合電化學感測器應用於偵測左旋多巴訊號。使用伏安法促使左旋多巴進行氧化反應，首先使用電化學感測器量測不同濃度的左旋多巴以確立伏安法特性，根據濃度與峰值電流值的線性迴歸曲線，可以計算出檢測極限為43.6 μM，略低於其他使用相似實驗架設的團隊的檢測極限。接著，我們將薄膜電晶體與電化學感測器連接，並量測相同濃度下的左旋多巴電流，藉由電晶體電流與電化學電流間的交互關係，形成一個訊號回饋的迴路，使得訊號穩定度提升。集成薄膜電晶體的電化學感測器的檢測極限為4.24 μM，相較於電化學感測器，可以達到更低的檢測極限。

關鍵字

薄膜電晶體；生物感測器；反應常數；左旋多巴；伏安法；回饋迴路

並列摘要

This paper introduces the different types and applications of biosensors composed of Indium-Gallium-Zinc-Oxide (IGZO) thin-film transistor (TFT). This research is divided into two parts: The first part is to use TFT combined with a linear shape microfluidic channel as the sensing platform, the chemical reaction kinetic between NADH (Nicotinamide Adenine Dinucleotide, reduced form), MDH (Malate dehydrogenase) and OAA (Oxaloacetic Acid, the conjugate acid of oxaloacetate) is the research target. First, different concentrations of NADH and NAD+ (Nicotinamide Adenine Dinucleotide) were injected into the microfluidic channel, and the current signal is monitored in real-time. And through the fitting of the diffusion model, the function of the drain current change with time was obtained. Next, we measured the NADH, OAA and MDH mixed solutions of different concentration multiples. Biochemical formulas and data results can provide information such as dissociation constants. It is worth noted that the derived equilibrium constant is (8.06 ± 0.61) × 104, which is similar to the research results of other teams. In the second part, the TFT is used as a negative feedback element and combined with an electrochemical sensor to detect the L-dopa (levodopa) signal. We used voltammetry to promote the oxidation reaction of L-dopa. First, an electrochemical sensor is to measure L-dopa at different concentrations to establish the voltammetric characteristics. According to the linear regression curve of the concentration and the peak current value, the detection limit can be calculated, which is 43.6 μM, slightly lower than the detection limit of other teams that used a similar setup. Next, we connected the TFT to the electrochemical sensor and measured the L-dopa current at the same concentration. The interaction between the TFT current and the electrochemical current forms a signal feedback loop, and the signal stability is improved. The detection limit of the electrochemical sensor with an integrated TFT is 4.24 μM, which value lower than the electrochemical sensor.

並列關鍵字

TFT ； biosensor ； reaction constant ； levodopa ； voltammetry ； feedback loop

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氧化銦鎵鋅薄膜電晶體結合電化學系統應用於生物化學反應之分析

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