經濟型掃描式電化學微探針系統的建立與紅血球介電泳的應用

Construction of an Economic Scanning Electrochemical Microprobe System and the Application on Dielectrophoresis of Red Blood Cells

施炫合(Syuan-He Shih)

指導教授 ： 陳力騏

國立臺灣大學/生物資源暨農學院/生物產業機電工程學研究所/碩士(2007年)

https://doi.org/10.6342/NTU.2007.01133

摘要

本研究旨在建構一套適合於顯微鏡下自由操控生物高分子或細胞的經濟型掃瞄探針系統，其組成包括自製微電極、3D掃描系統與其使用者介面，以及可供直接觀察用的介電泳槽。
微電極的製作利用直徑0.5mm的鉛筆芯以電化學的方式於3Vrms交流電壓於1M NaOH溶液蝕刻9分鐘，並將蝕刻完的鉛筆芯以快速硬化劑將鉛筆芯封裝於玻璃毛細管中。最後再以鑽石砂紙研磨使微電極具微米大小的電活性區域露出(半徑約10μm)。以此方式製作的微電極的電化學特性近似於一般黏土碳微盤形電極。
3D掃描系統利用步進馬達以LabVIEW

關鍵字

微鉛筆芯電極 ； 鉛筆芯蝕刻 ； 紅血球細胞介電泳操控 ； 經濟型細胞分子操控平台

並列摘要

An economic scanning probe system has been constructed for manipulating biomolecules and/or cells under microscopic observation; the system included a home-made microelectrodes, PC-controlled 3D-scanning system, the user interface and a dielectrophoresis chamber for direct observation.
The micro-electrode was made by electrochemical etching (3Vrms for 9mins in 1M NaOH) of a pencil lead (0.5mm in diameter), and then the sharpened pencil lead was sealed (insulated) within a glass capillary using cyanoacrylate adhesive. The electrode tip was polished with diamond lapping films to reveal its micro-scaled electroactive region (~10μm in diameter). Its electrochemical characteristics are similar to a commercial carbon paste micro-disk electrode.
The 3D-scanning system was driven with three stepped motors controlled by a user interface program written under Labview

並列關鍵字

Microelectrodes ； Dielectrophoresis ； Cell manipulation ； Indium tin oxide

參考文獻

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