- 學位論文
以柴油培養之Rhodococcus erythropolis NTU-1結塊處理水包油(O/W)型乳化液
De-emulsification of oil-in-water emulsion by Rhodococcus erythropolis NTU-1 bioflocculi grown in diesel
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摘要
NTU-1是從石油汙泥中分離並透過16S rDNA序列鑑定確立其所屬菌屬為Rhodococcus erythropolis。經由培養、烘乾後，可得到NTU-1結塊之粉末，此粉末具有很好的破乳化能力。 本研究比較以柴油培養的NTU-1粉末及以正十六烷培養的NTU-1粉末之破乳化效率。經實驗結果發現，以柴油培養的NTU-1粉末具有生產成本較低、使用量較少以及破乳化能力較佳之優勢。若以柴油培養的NTU-1粉末進行破乳化測試，其最適添加量與Tween-80體積存在著三分之二次方的關係。於O/W/T比例為4/1/0.01，在480分鐘時油相回收百分比可達95%。此外，當乳化液的油/水比(O/W)較大時或連續相黏度較低時，可提升油相回收百分比。細胞表面疏水性是影響破乳化重要的因素。實驗結果顯示，微生物細胞疏水性與油相回收百分比呈現正相關。 綜括上述，以烘乾後的NTU-1粉末處理水包油(O/W)型乳化液具有相當的潛力以及發展性。
並列摘要
NTU-1, a highly efficient demulsifying strain, was isolated from petroleum contaminated soil and identified as Rhodococcus erythropolis by 16S rDNA gene analysis. NTU-1 powder with high demulsifying performance could be obtained by heat-drying after collecting bioflocculi in medium. This study first evaluated the demulsifying performance of NTU-1 cultured by diesel and n-hexadecane, and found that NTU-1 powder by diesel is a better choice for its lower cost and better performance. At an optimal quantity of dried NTU-1 powder for de-emulsification that was proportional to the volume of Tween-80 to the 2/3 power, a recovery of 95 % oil was achieved in 480 min. This was made possible when the oil/water/Tween80 volume ratio was set at 4/1/0.01. The higher O/W ratio and the lower viscosity both contributed to such a high oil recovery rate. The positive correlation between CSH (MATH) and oil recovery ratio also implies that the diesel grown NTU-1 culture may provide a commercial scale-up method to break oil -in-water emulsion directly at the primary oil recovery site.
參考文獻
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被引用紀錄
張逸安（2016）。以Rhodococcus erythropolis NTU-1復育受正十六烷污染之土壤〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU201601878
延伸閱讀
- 施文宗（2005）。生質柴油混合水乳化柴油之燃料應用於直噴式柴油引擎之研究〔碩士論文，國立臺北科技大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0006-2208200515541800
- 胡苔莉、李其靜（2014）。利用有機廢水培養Chlorella protothecoides產製生質柴油可行性評估。中華生質能源學會會誌，33(3&4)，97-103。https://www.airitilibrary.com/Article/Detail?DocID=P20170119002-201412-201704140010-201704140010-97-103
- 黃晟硯（2007）。廢食用油製造生質柴油(Biodiesel)-新式預酯化反應流程之參數探討〔碩士論文，國立清華大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0016-1411200715095980
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- Wu, K., Li, W., Song, J., & Li, T. (2015). Production, Purification, and Identification of Cholest- 4-en-3-one Produced by Cholesterol Oxidase from Rhodococcus sp. in Aqueous/Organic Biphasic System. Biochemistry Insights, 2015(S1), 1-8. https://doi.org/10.4137/BCI.S21580