深層培養生產具有維護肝臟功能之樟芝發酵產物

Submerged cultivation of Antrodia cinnamomea for manufacturing the fermentation products possessing liver protective function

陳乙菘(Yi-Song Chen)

指導教授 ： 沈立言

共同指導教授 ： 蔣丙煌(Been-Huang Chiang)

國立臺灣大學/生物資源暨農學院/食品科技研究所/博士(2008年)

https://doi.org/10.6342/NTU.2008.01362

摘要

本研究評估並建立可生產具有抑制人類肝癌細胞活性與護肝功能特性的樟芝（Antrodia cinnamomea, AC)深層發酵條件，結果發現搖瓶發酵8週的樟芝菌絲體乙醇萃取物具有較佳抑制Hep3B生長的活性，其中以glucose為碳源發酵8週的樟芝菌絲體乙醇萃取物（AC8-Em-glu）對Hep3B生長抑制的IC50最低，為76.2

關鍵字

樟芝 ； 菌絲體 ； 人類肝癌細胞 ； 初代肝細胞 ； 四氯化碳 ； 活性成分

並列摘要

The objective of this research was to establish proper fermentation conditions for submerged cultivation of Antrodia cinnamomea (AC) to produce a fermentation product with antihepatoma activity. AC was fermented for 1, 2, 4, 6 and 8 weeks with galactose, lactose and glucose as carbon sources of the submerged cultivation medium in 250 ml flask. The cell viability of Hep3B was more inhibited with the treatment of ethanolic extract of AC mycelia fermented for 8 weeks than those fermented for 1, 2, 4 and 6 weeks. The IC50 of ethanolic extract of AC mycelia fermented for 8 weeks with glucose as a carbon source (AC8-Em-glu) was 76.2

並列關鍵字

A. cinnamomea ； mycelia ； hepatoma cell line ； primary rat hepatocytes ； CCl4 ； activity compounds

參考文獻

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