幾丁聚醣作為藥物釋放和組織工程上的應用

The Application of Chitosan in Drug Delivery and Tissue Engineering

李啟榮(Chi-Jung Lee)

指導教授 ： 黃義侑

國立臺灣大學/工學院/醫學工程學研究所/碩士(2004年)

https://doi.org/10.6342/NTU.2004.01383

摘要

本研究以HPC/CHOL=70/30(molar ratio)脂質組成200nm大小微脂粒。利用微脂粒包覆樣本藥物indomethacin及trypsin，並在37°C下將之分散於幾丁聚醣凝膠中以探討藥物釋放的情形及其機制。實驗結果顯示經由微脂粒包覆之樣本藥物在幾丁聚醣凝膠中可減緩其釋放速率，證實凝膠和微脂粒結合的藥物釋放系統具有絕佳的控制釋藥能力，尤其以去乙醯化程度95%的幾丁聚醣凝呈現較佳。利用共焦顯微影像系統分析螢光球在幾丁聚醣凝膠中滲透深度，可知去乙醯化程度95%的幾丁聚醣滲透較淺，可更有效的減緩其釋放速率。且由於幾丁聚醣具有良好的生物相容性，輪部幹細胞能吸附在幾丁聚醣薄膜上生長，因此做為細胞載體是一可行的方向，而本研究結果也顯示幾丁聚醣凝膠在藥物釋放及組織培養皆有良好的潛能。

關鍵字

藥物釋放 ； 微脂粒 ； 組織工程 ； 幾丁聚醣

並列摘要

Liposomes with particle size 200nm, composed of hydrogenated PC (HPC) and cholesterol ( mole ratio = 70:30 ), were used in this study. The mechanisms by using liposomes encapsulating trypsin and indomethacin dispersed in chitosan gel at 37℃as a drug delivery system were investigated. Experimental results showed that drug-loaded liposomes within chitosan gel can not only form a homogeneous system but reduce the release rate of drugs, which indicated that gel-liposome drug release system possess excellent controlled release ability. Among all experimental conditions, chitosan with 95% deacetylation degree showed the best sustain release effect. Making use of Confocal Laser Scanning Microscopy to measure the penetration depths of Fluorescence Microspheres in chitosan gel, it can be found that Fluorescence Microspheres in 95% deacetylated chitosan gel permeated less and therefore effectively decelerate the release. For chitosan being a well biocompatible material, limbus stem call can be loaded and cultured on chitisan-membrane, which lead to a new research area for chitosan-membrane to be a cell-carrying substrate. Generally, study results demonstrated that chitosan gel materials have great potential in both drug delivery and tissue culture.

並列關鍵字

Liposome ； Chitosan ； Drug delivery ； Tissue engineer

參考文獻

[1] Collins-Gold L.C., Lyons R.T., Batholow L.C., Parenteral Emulsions for Drug Delivery, Adv. Drug Deliv. Rev. 5, p.189-208, 1990

[2] Sharma A., Sharma U.S., Liposomes in Drug Delivery : Progress and Limitations, Int. J. Pharm. 154, p.123-140,1997

[3] Hatefi A.,Amsden B., Biodegradable Injectable in Situ Forming Drug Delivery Systems. Journal of Controlled Release. 80, p.9-28, 2002

[4] DiTizio V., Karlgard C., Lilge L., Khourt A.E., Mittelman M., Localized Drug Delivery Using Crosslinked Gelatin Gels Containing Liposomes : Factors Influencing Liposome Stability and Drug Release. Inc. J Biomed Mater Res, 51, p.96-106, 2000

[6] Bochot A., Fattal E., Grossiod J.L., Puisieux F. Couvreur P., Characterization of A New Ocular Delivery System Based on A Dispersion of Liposomes in A Thermosensitive Gel. International Journal of Pharmaceutics. 162, p.119-127 1998