- 學位論文
聚乳酸-聚甘醇酸之共聚高分子塗佈於聚四氟乙烯引導韌帶修復之研究
The study of guided ligament regeneration by using e-PTFE coated with PLGA
摘要
我們利用組織工程的方法來引導韌帶的再生。首先，我們將紐西蘭白兔的前十字韌帶細胞及內側附韌帶細胞培養在聚乳酸-聚甘醇酸之高分子共聚物(PLGA)及聚四氟乙烯(e-PTFE)來檢測細胞在此兩種材料上的活性。第二，為了檢測此兩種材料的生物適合性，我們將此兩種材料植入老鼠的背部。第三，我們將聚四氟乙烯纖維塗佈聚乳酸-聚甘醇酸作成支架，聚四氟乙烯可以提供韌帶初期修復時所需的支撐力，聚乳酸-聚甘醇酸可以提供細胞生長的空間。最後我們在體外利用此種支架架接紐西蘭白兔的前十字韌帶。 MTT檢測的結果證實韌帶細胞在聚乳酸-聚甘醇酸上的生長情形良好。在細胞-材料系統中，細胞在聚乳酸-聚甘醇酸及細胞培養皿上有正常的細胞型態，但在聚四氟乙烯卻無正常的細胞型態。另外，當韌帶組織被固定在聚乳酸-聚甘醇酸上培養時，細胞會從韌帶組織向外長出並遷移至細胞培養皿，但韌帶組織被固定在聚四氟乙烯上培養時，細胞不會從韌帶組織向外長出並且也不會遷移至細胞培養皿。植入實驗，此兩種材料不會引起嚴重的發炎反應，而且纖維母細胞會沿著網狀聚四氟乙烯的隙縫生長。架接結果顯示細胞會從斷端的韌帶組織遷移到支架並且貼附良好。這些實驗數據支持我們的想法，可以利用聚四氟乙烯塗佈聚乳酸-聚甘醇酸來架接韌帶。聚四氟乙烯提供初期的支撐力量，聚乳酸-聚甘醇酸提供細胞生長空間，最後，細胞生長進入該支架內並分泌細胞外基質，以細胞外基質取代支架，提供未來得支撐力。 總結，利用聚四氟乙烯塗佈聚乳酸-聚甘醇酸所做成的支架可以提供初期的支撐力及細胞的生長空間，因此利用此種支架來架接韌帶將有其可性。
並列摘要
In this study, we had a purpose on tissue engineering for guiding ligament regeneration. First, we investigated Poly (lactic-co-glycolic acid) (PLGA) and expanded poly (tetrafluoroethylene) (e-PTFE) viability by culturing both New Zealand white rabbit anterior cruciate ligament (ACL) cells and medial collateral ligament (MCL) cells on these materials. Second, we investigated PLGA and e-PTFEs’ compatibility with tissue by implanting subcutaneously e-PTFE web coated with PLGA into the back of rats. Third, we fabricated a new scaffold that consisted of PLGA and e-PTFE scrim yarns. PLGA could provide a space for cells growth and e-PTFE scrim yarns could provide a force to resist the strength during initial ligament repairing period. Finally, we use this new scaffold to bridge the rupture New Zealand whit rabbit ACL in vitro. MTT (3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyl tetrazolium bromide) assay showed ligament cells could proliferate and grow well on PLGA membrane. In cells-material culturing system, we also observed that ligament cells did not present their normal cell morphology on the expanded poly (tetrafluoroethylene) (e-PTFE) film but cells presented their normal morphology on PLGA and tissue culture polystyrene (TCPS). In addition, ligament cells could migrate to TCPS from ligament explant that was put onto PLGA membrane, but ligament cells could not migrate to TCPS from the ligament explant that was put on e-PTFE film. In implantation result, either material did not induce seriously inflammatory reaction and the fibroblast cell could grow into the gap of e-PTFE web. The result of guided ACL regeneration showed that the ACL cell could migrate to the new scaffold from the rupture end of ACL explant. These results support our hypothesis that e-PTFE provided initial force to resist the strength during the ligament-repairing period and eventually was replaced by the surrounding extracellular matrix (ECM), which was produced by in-growth cells. Finally, the ECM could provide mechanic force. To sum up, the new scaffold, made of e-PTFE scrim yarns coated with PLGA, had a pore size ranged from 10~50mm and this pore size was good for fibroblast cell growing. Hence, e-PTFE scrim yarns coated with PLGA could be used to develop a new scaffold for guiding ligament regeneration in the future.
參考文獻
延伸閱讀
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