- 學位論文
Lactobacillus paracasei subsp. paracasei NTU 101 乳酸菌菌粉對經由雙側總頸動脈斷流再灌流手術誘導之腦中風大鼠學習記憶能力改善效果
Effect of Lactobacillus paracasei subsp. paracasei NTU 101 powder to improve memory and learning ability on stroke rats induced by bilateral common carotid artery occlusion-reperfusion
摘要
腦中風再灌流 (cerebral stroke reperfusion) 所導致腦部傷害包括腦中產生大量自由基所造成氧化壓力傷害、能量不平衡、發炎反應以及細胞凋亡。先前多篇文獻指出乳酸菌具有降低氧化壓力、發炎反應等功效，另外近年也有文獻提到益生菌透過菌腦腸軸線 (brain-gut-microbiota axis) 間接影響腦部認知功能。本研究探討 Lactobacillus paracasei subsp. paracasei NTU 101 原末菌粉對腦中風大鼠是否具有改善學習記憶能力及降低腦中發炎反應的效果。腦中風的動物誘導模式使用八週齡大之 Sparque-Dawley (SD) 雄性大鼠進行雙側總動脈斷流再灌流 (bilateral common carotid artery occliusion-reperfusion, BCCAO) 手術。此誘導模式會造成大鼠學習記憶能力大幅下降、腦部嚴重發炎，而餵食 NTU 101原末菌粉對大鼠學習記憶能力具有明顯的改善效果，可抑制神經膠質細胞 (neuroglial cell) 中星形膠質細胞 (astrocyte) 及微膠細胞 (microglia) 的活化，並降低腦中發炎細胞激素 TNF (tumor necrosis factor)-α、IL (interleukin)-6和 IL-1β及一氧化氮 (nitric oxide) 濃度，顯著降低發炎相關蛋白 iNOS (inducible nitric oxide synthase)、NF-κB以及MAPK路徑蛋白之表現量。綜合以上結果，餵食 NTU 101原末菌粉可透過減輕發炎反應，改善腦中風再灌流所造成的學習記憶損傷。
並列摘要
Cerebral stroke reperfusion caused brain injury including a large number of free radicals generated in the brain caused by oxidative stress damage, energy imbalance, inflammation and apoptosis. Many papers previously mentioned that lactic acid bacteria (LAB) can reduce oxidative stress, inflammation and other effects. On the other hand, in recent years, probiotics affecting cognitive function of the brain through the brain-gut axis indirectly have been reported. This study investigated whether the Lactobacillus paracasei subsp. paracasei NTU 101 improves learning and memory and reduces brain inflammation in stroke rat. In this research, all male Sprague-Dawley rats accept bilateral common carotid artery occlusion-reperfusion (BCCAO) operation to induce memory and learning ability impairment of stroke. This animal model leaded to learning and memory impairment and a serious inflammation of the brain, while NTU 101 administration improved cognitive impairment. Additionally, NTU 101 inhibited astrocytes and microglia activation, therefore reduced proinflammatory cytokines including tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-1β and nitric oxide (NO). Moreover, the expression of inflammatory-related proteins inducible nitric oxide synthase (iNOS), NF-κB and protein of MAPKs signaling pathway were decreased in NTU 101-treated groups. In summary, administration of NTU 101 can against the impairment of memory and learning ability induced by stroke via ameliorating inflammation.
參考文獻
延伸閱讀
- 許培祐（2016）。Lactobacillus paracasei subsp. paracasei NTU 101 乳酸菌粉對 dextran sulfate sodium (DSS) 誘導小鼠結腸炎的改善效果〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU201602274
- Chan, C. J. (2017). 探討繫帶水解物在D-半乳糖誘導老化小鼠模式下對認知行為障礙及腦中氧化傷害之保護功效 [master's thesis, National Taiwan University]. Airiti Library. https://doi.org/10.6342/NTU201700417
- Lin, C. L. (2008). 二氫基女性素與腺苷酸A2A接受器促效劑治療蜘蛛膜下腔出血引發的腦血管痙攣與腦損傷之病理機轉與治療探討 [doctoral dissertation, Kaohsiung Medical University]. Airiti Library. https://doi.org/10.6832/KMU.2008.00022
- Wei, L. S. (2011). 第一部分 龍眼花萃取物對果糖餵養大鼠抗心臟凋亡分子機轉之探討 第二部分 Diosgenin對D-半乳糖誘導老化大鼠心臟凋亡和纖維化之探討 [master's thesis, Asia University]. Airiti Library. https://www.airitilibrary.com/Article/Detail?DocID=U0118-1511201215471343
- 劉曲婷（2010）。Effects of Lactobacillus casei 01 and its exopolysaccharide on 1,2-dimethylhydrazine induced colon precancerous lesions in F344 rats〔博士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2010.03268