- 學位論文
連續式非酵素葡萄糖感測器可行性研究
Feasibility Study on Continuous Nonenzymatic Sensors for Glucose Sensing
若您是本文的作者，可授權文章由華藝線上圖書館中協助推廣。
摘要
人體中葡萄糖濃度(Glucose concentration)是糖尿病治療、診斷與監控的黃金指標。傳統上所使用的葡萄糖感測器，是利用葡萄糖與電極上的酵素結合，透過化學反應產生與葡萄糖濃度成正比的電流來感測。然而傳統酵素感測器除了會遇到製程和保存上的困難，酵素在人體內無法長期保存而人體被代謝。 本研究中，嘗試用非酵素式且具有高度生物相容性的材料Pt-Ir 取代傳統酵素型的葡萄糖感測器，希望未來能應用於連續式葡萄糖監控(continuous glucose monitoring, CGM)。本研究分為兩階段，第一階段為Pt-Ir葡萄糖感測器製程及功能測試，內容包含由泰博公司所提供的葡萄糖感測器的電極，透過後製程和穩定性開發測試以及感測器特性測試(sensitivity and selectivity)，並透過高密度恆電流(galvanostatic)電鍍的方式，造成工作電極(working electrode)的奈米結構呈現葉片狀(leaf-like)來增加催化反應面積(catalytic reaction area)。第二階段為動物實驗，以小鼠為實驗標的，測試感測器的穩定性及植入後使用時間的表現。
並列摘要
Glucose concentration in human bodies is gold criteria for Diabetes Mellitus of diagnosis and treatment. The conventional glucose sensors are utilized enzyme electrode to sensing glucose moleculars. However, the conventional enzyme glucose sensors are difficult to synthesis , store and long-term stay in human bodies. In this research, we attempt using non-enzymatic platinum-Iridium(Pt-Ir) glucose sensor to replace conventional glucose sensors and look forward to applicate in continuous glucose monitoring(CGM). The research were separated into two parts, the first is the fabrication of platinum-Iridium glucose sensor and its feasibility test, including sensitivity, linearity and interference test. The substrate of the electrode is come from TaiDoc Technology and then using galvanostatic method to electroplate the nano-structure of Pt-Ir. The leaf-like of the nano-structure is believed that can increase the catalytic reaction area. The second part is the animal test, the electrodes were implanted into the subcutaneous of mice to test the stability and life cycle.
參考文獻
[3] C.D Mathers, and D.Loncar "Projections of global mortality and burden of disease from 2002 to 2030",Plos med.3(2006): e442
[4]The effect of intensive treatment of diabetes on the development and progression of long-term complications in insulin-dependent diabetes mellitus",N Engl J Med.329(1993): 977-986
[5] J. Brauker, "Continuous glucose sensing: future technology developments",Diabetes technology & therapeutics.11(2009): S-25-S-36
[6] S.P. Nichols , et al., "Biocompatible materials for continuous glucose monitoring devices",Chemical reviews.113(2013): 2528-2549
[7] B. Feldman, et al., "A continuous glucose sensor based on Wired Enzyme™ technology-Results from a 3-day trial in patients with type 1 diabetes",Diabetes technology & therapeutics.5(2003): 769-779