We used the ITO film as the buffer layer to grow CdSSe quantum dots on glass substrate. And we also observed the annealing effects on CdSSe quantum dots light emitting. The first of all , we changed the thermal annealing temperature and time to find out the best process condition. Then we used the ITO as the capped layer to cover the CdSSe quantum dots. We can use the AFM to find out the best coverage of ITO.At last we observed the photoluminescence of ITO/CdSSe/ITO/glass heterostructure. Because of lattice dialation ,the photoluminescence of CdSe quantum dots has blue shift.