幾丁類物質於色素吸附之利用

靈菌紅素(prodigiosin；PG)具有諸如抗癌、抗菌以及殺蟲等生物活性。本研究針對Serratia marcescens TKU011發酵烏賊軟骨生產靈菌紅素之條件進行探討。結果發現磷酸鹽會抑制靈菌紅素的生產。此1.5％烏賊軟骨培養基經高壓滅菌後，添加50mL蒸餾水於離心所得沉澱物作為培養基，於30℃發酵三天所得靈菌紅素產量 (2.38mg/mL)提升約2.5倍。添加乳酸菌、棉花或木棉花於1.5%烏賊軟骨培養基，探討藉由這些添加物之吸附作用，增加靈菌紅素產量之可行性。結果顯示添加0.1%（w/v）木棉花所得靈菌紅素產量(1.30mg/mL)為未添加（0.26mg/mL）之5倍。至於與烏賊軟骨同屬幾丁類物質的蝦頭、蟬蛻以及幾丁質之效果亦有探討，其中以乳酸菌和蟬蛻粉吸附靈菌紅素的效果最好。靈菌紅素屬脂溶性色素，因此亦探討上述幾丁類物質對於紅色40號（R40）或黃色4號（Y4）水溶性食用色素之吸附效果。結果顯示烏賊軟骨的效果最好，對於紅色40號及黃色40號之吸附率分別為14mg/g及12.3mg/g。此外，亦探討粒徑大小、去乙醯度、去蛋白質以及色素酸鹼度等條件對於吸附效果之影響。界面活性劑SDS可將烏賊軟骨所吸附色素幾乎完全都脫附下來，最具重複使用之潛力。

關鍵字

靈菌紅素；沙雷氏菌；乳酸桿菌；幾丁質；食用色素

並列摘要

Prodigiosins are known to exert antitumor, antibacterial and insecticidal activities. Using fishery-processing wastes of squid pen power（SPP）as the sole carbon and nitrogen（C/N）source, Serratia marcescens TKU011 produced prodigiosin. The relationship between prodigiosin production and phosphate concentration was studied. The medium（1.5％SPP、0.05％MgSO4•7H2O、0.1％K2HPO4） was sterilized and centrifuged, and the deposits were collected and supplied 50ml water. Production in the medium at 30 ℃ for 3 days showed highest level of prodigiosin production（2.38mg/mL）. Lactic acid bacteria, cotton, and Bombax ceiba have been tested for their enhancing effects on prodigiosin production of S. marcescensTKU011 by supplementing the cells and adsorbents into SPP-containing medium. Bombax ceiba showed best results of increasing 5-folds of prodigiosin productivity compared with the non-supplement group. Among SPP and other chitinous materials of cicada casting powder（CCP）, shrimp shell power（SSP）and chitin, lactic acid bacteria and CCP showed best adsorption on prodigiosin. As for the adsorbing effects of water-soluble food colorants of allura red AC（R40）and tartrazne（Y4），SPP showed the best adsorption. The capacities of SPP adsorption for R40 and Y4 were determined to be 14mg/g and 12.3 mg/g respectively. Regeneration experiments showed that the water-soluble pigments can be completely regenerated with surfactant-SDS solution.

並列關鍵字

prodigiosin ； Serratia marcescens ； Lactobacillus ； chitin ； food colorant

參考文獻

Alihosseini F, Ju KS, Lango J, Hammock BD, Sun G（2008） Biotechnology Progress,24：742-747

Clements Jewery S.（1976）The reversal of glucose repressed prodigiosin production in Serratia marcescens by the cyclic 3`, 5`adenosine monophosphate inhibitor theophylline. Experientia, 32:421–422.

Copello GJ, Mebert AM, Raineri M, Pesenti MP, Diaz LE（2011）Removal of dyes from water using chitosan hydrogel/SiO2 and chitin hydrogel/SiO2 hybrid materials obtained by the sol-gel method. Journal of Hazardous Materials,186：932-939

De Araujo HWC, Fukushima K, Takaki GMC（2010）Prodigiosin production by Serratia marcescens UCP 1549 using renewable-resources as a low cost substrate. Molecules, 15:6931-6940.

Dolphen R, Sakkayawong N, Thiravetyan P, Nakbanpote W（2007）Adsorption of reactive red 141 from wastewater onto modified chitin. Journal of Hazardous Material, 145：250-255

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幾丁類物質於色素吸附之利用

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