Burkholderia cepacia TKU026 發酵烏賊軟骨生產殺蟲劑和酪胺酸酶抑制劑之研究

Studies on the production of pesticides and tyrosinase inhibators by Burkholderia cepacia TKU026 fermentation on squid pen

陳勇為(Yung-Wei Chen)

指導教授 ： 王三郎

淡江大學/理學院/化學學系碩士班/碩士(2013年)

https://doi.org/10.6846/TKU.2013.01001

摘要

Burkholderia cepacia TKU026為以烏賊軟骨為唯一碳/氮源，篩選自台北市內湖土壤之酪胺酸酶抑制劑生產菌。本研究針對此菌發酵所得上清液之果蠅致死率以及酪胺酸酶抑制劑活性測試。果蠅外殼所含幾丁質係幾丁質合成酶所生成，而文獻報導幾丁質酶抑制劑和酪胺酸酶抑制劑具類似性質，因而期待藉由酪胺酸酶抑制劑抑制果蠅外殼幾丁質之形成而達到殺蟲效果。
結果顯示較適培養條件為，將菌株B. cepacia TKU026接種於50 mL含有1 %烏賊軟骨粉、0.1 % K2HPO4及0.05 % MgSO4．7H2O (pH 7) 之液體培養基（裝填於250 mL震盪三角瓶加上通氣栓）於37℃經搖瓶(150 rpm) 培養2天，可具有較佳殺果蠅和酪胺酸酶抑制效果，分別為81%和95%。本實驗同時探討此殺蟲劑和酪胺酸酶抑制劑之性質與分離純化，結果指出，此物質應該為小分子化合物，具有耐熱、穩定性高的特性。

關鍵字

Burkholderia cepacia ； 酪胺酸酶抑劑 ； 烏賊軟骨 ； 殺蟲劑

並列摘要

Burkholderia cepacia TKU026, a tyrosinase inhibitor-producing strain, was isolated from Taipei soil using squid pen as the sole carbon/nitrogen source. B. cepacia produced higher inhibitory activity (95%) when the organism was incubated in a baffle-based flask containing 50 mL medium of 1% squid pen powder, 0.1 % K2HPO4, and 0.05 % MgSO4 •7H2O (pH 7) at 37℃ for two days.
Some tyrosinase inhibitors with chitinase inhibitory activities have been investigated their insecticidal activities. In the present study, the use of tyrosinase inhibitor as insecticide was also studied. Results showed that Drosophila larval motility of the tyrosinase inhibitor was 81%. Based on the results of dialysis and heat-treatment, the inhibitor was inferred to be a thermostable compound of low molecule.

並列關鍵字

Burkholderia cepacia ； tyrosinase inhibitor ； squid pen ； insecticide

參考文獻

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