香瓜茄水萃物對於以高脂飲食合併STZ誘發糖尿病小鼠之影響

The effect of Solanum muricatum Ait aqueous extract on high-fat diet and streptozotocin induced diabetic mice

蕭亭萱(Tyng-Shiuan Hsiau)

指導教授 ： 徐成金

中山醫學大學/健康管理學院/營養學系碩士班/碩士(2014年)

https://doi.org/10.6834/CSMU.2014.00072

摘要

高血糖會促使過多葡萄糖走向多元醇路徑代謝，增加體內氧化壓力，加重體內處於慢性發炎的狀態。在本實驗室先前的研究發現，台灣澎湖地區特產的茄科茄屬植物香瓜茄，對第2型糖尿病小鼠具有延緩體內傷害及抑制多元醇路徑之作用。因此本研究更進一步以高脂飲食合併STZ為策略，誘發BALB/c小鼠為第2型糖尿病的進程，再分別以1%和2%香瓜茄水萃物餵食6週後犧牲並分析，觀察對於小鼠第2型糖尿病的影響。結果顯示，餵食香瓜茄水萃物的組別與糖尿病組相比，血糖、胰島素阻抗與血脂異常皆有顯著的改善；在多元醇路徑方面，其關鍵酵素醛糖還原酶(aldose reductase)及其終產物fructose和CML在腎臟中皆有下降；在抗氧化方面，肝臟中氧化壓力有顯著降低、抗氧化酵素活性增加，且肝臟、腎臟和胰臟的總抗氧化能力亦有增加，能降低氧化傷害；由脂肪細胞所分泌，與體內發炎反應相關的細胞激素TNF-α、IL-1β和IL-6亦有下降情形；且能活化PPARγ、增加gastric inhibitory polypeptide (GIP)分泌。因此本研究結果證實，香瓜茄水萃物具有降低高脂飲食與STZ誘發糖尿病小鼠體內的氧化壓力、糖化作用和發炎反應的效果，能預防糖尿病相關併發症的發生。

關鍵字

高脂飲食 ； 第2型糖尿病 ； 香瓜茄 ； 多元醇路徑 ； 發炎反應

並列摘要

Hyperglycemia from diabetes promotes glucose to polyol pathway flux, which in turn causes tissue damage through oxidative and inflammatory stress. Solanum muricatum Ait (pepino) is a popular plant food in Penghu, Taiwan. Our previous study has reported that the aqueous extract from pepino was able to enhance antioxidant defense and decline polyol pathway in streptozotocin induced type 2 diabetic BALB/c mice. Therefore, we further used high-fat diet and streptozotocin induced type 2 diabetic BALB/c mice as a model to investigate the protective effects of pepino aqueous extract. Diabetic mice were fed by 1 or 2% aqueous pepino for six weeks. The serum and tissues were collected to analyze. Results showed that pepino intake improved hyperglycemia, insulin resistance and dyslipidemia. This extract decreased the activity of aldose reductase, a key enzyme in polyol pathway in kidney. Also, the accumulation of glycoxidation products, fructose and N-ε-carboxymethyl-lysine levels were reduced. In addition, this extract significantly reduced malondialdehyde level, retained glutathione content and glutathione peroxidase activity in liver. Pepino intake also increased total antioxidant capacity in liver, kidney and pancreas, attenuated oxidative damage. Proinflammatory cytokines such as TNF-α、IL-1β and IL-6 produced by adipose tissue were also decreased. In addition, pepino intake up-regulated PPARγ and increased gastric inhibitory polypeptide secretion. These results suggested that pepino aqueous extract could prevent progression of high-fat diet and streptozotocin induced type 2 diabetic complications by decreasing oxidative stress, glycosylation and inflammatory response.

並列關鍵字

high-fat diet ； type 2 diabetes ； Solanum muricatum Ait ； polyol pathway ； inflammatory response

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