線上固相萃取結合液相層析串聯質譜儀進行多重濫用藥物同時分析之研究與探討

Simultaneous Determination of Multiple Drugs of Abuse using On-line Solid Phase Extraction coupling with Liquid Chromatography Tandem Mass Spectrometry

洪維胤(Wei-Yin Hong)

指導教授 ： 張耀仁

中山醫學大學/醫學院/醫學研究所/碩士(2012年)

https://doi.org/10.6834/CSMU.2012.00081

摘要

雖然時代不斷進步，新科技與新產品帶給人們更多的生活樂趣，但不管在台灣或全世界，濫用藥物的氾濫情形卻越來越嚴重。近年來除了常見的濫用藥物外，更多種類的新興濫用藥物不斷的出現，使用新的檢驗方法，特別是靈活且高效率的多重濫用藥物檢驗，更是目前發展的主流。因此本研究首先成功的建立尿液中55種多重濫用藥物液相層析串聯質譜（LC-MS/MS）檢測平台，然而，因前處理部分，採用液相-液相萃取方式極為繁瑣費時，隨後即改良成『線上固相萃取 (on-lineSPE) 』，並進行方法的確效，包含偵測極限、定量極限、線性範圍、回收率、基質效應、精密度與準確度之評估。最後將方法用於真實檢體之分析。由於 on-line SPE LC-MS/MS 具有極佳的藥物擴充性與檢體適用性，未來可以不斷的針對新興藥物，擴大分析藥物範圍，也可將此分析平台應用至如唾液、毛髮等更多的生物檢體上。

關鍵字

線上固相萃取 ； 多重濫用藥物 ； 液向層析串聯質譜儀 ； 尿液

並列摘要

Drugs of abuse is a serious problem that is continuously and progressively increasing over the world. As time progresses, the classes of the drugs consumed are steadily increasing, and monitor subjects must be continuously extended. Therefore, it is emergent to the development of simultaneously analysis for multiple drugs of abuse. This study presents a liquid-liquid extraction (LLE) coupled with the liquid chromatography method coupled with tandem mass spectrometry (LC-MS/MS) method that was developed for determining multiple drugs in human urine. The pre-treatment is too long time, so we developed an on-line solid phase extraction (SPE) method. The limit of quantitation (LOQ) range is 0.5-20 ng/mL and the limit of detection (LOD) is 0.1-5 ng/mL. The current method was applied to determine of 45 authentic urine specimens to prove the practicability in routine analysis. The whole procedure was applied in the analysis of urine of patients positive to the test.In the future, the on-line SPE LC-MS/MS method will been evaluated the drugs in human oral fluid and hair.

並列關鍵字

on-line SPE ； drugs of abuse ； LC-MS/MS ； urine

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