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  • 學位論文

蔓越莓汁之體外生物活性及調節人體血脂之研究

Effects of cranberry juice on the in vitro biological activities and human blood lipid regulation

指導教授 : 王進崑 陳肅霖

摘要


本研究採用蔓越莓汁及其三種區分物包括n-hexane、ethyl acetate (EA)和高極性(hi polar)區分物,進行酚類化合物之特性探討、抗氧化性、自由基清除活性及cyclooxygenase 2 (COX-2)表現之抑制等生物活性測定。結果顯示,蔓越莓汁之酚類化合物主要存在於EA區分物中,蔓越莓汁及其區分物皆具有抗氧化性、自由基清除活性以及抑制COX-2活性之功效,且隨劑量呈正相關,而以EA區分物的效果最佳。 本研究 in vitro試驗證實蔓越莓汁具有抗氧化性,因此以臨床試驗探討飲用蔓越莓汁後,人體之抗氧化性表現及其在血脂調整之貢獻 。本試驗採隨機、安慰劑對照之設計,共計40人參與。實驗組之受試者每天飲用含有27%之蔓越莓汁660mL,而對照組受試者則給予660mL不含蔓越莓汁成份,但特別調製與試驗組風味相似且含等量維生素C之飲料。本實驗共進行14週,試驗前後2週分別為穩定期與觀察期,試驗期為10週。結果顯示,兩組受試者之體重、體脂肪、身體質量指數在試驗前後並無顯著變化。在抗氧化性方面,飲用蔓越莓汁連續6週以上時,血漿之脂質過氧化程度(TBARS值)顯著(p<0.05)地低於對照組,同時血漿之總抗氧化性、血漿中維生素C、含硫官能基含量顯著高於穩定期,並達到統計意義。此外,連續飲用10週時,實驗組LDL之氧化延滯期顯著(p<0.05)高於對照組;由本研究可知,每天飲用27%蔓越莓汁660mL並且連續飲用10週,確實可有效增加人體之抗氧化能力並增加LDL之氧化耐受性,而主要之貢獻成分可能是蔓越莓汁中的酚類化合物。

關鍵字

蔓越莓 抗氧化性 LDL 自由基清除 COX-2

並列摘要


This study was focused on the biological activities of cranberry juice and its various fractions, and also the effect on antioxidant activity and blood lipid regulation in human body. Three fractions of cranberry juice were used in this study, including n-hexane, ethyl acetate(EA)and high polarity fractions (continuous extraction by the order of n-hexane and EA ). Reducing activity, chelating efficiency on ferrous ion, scavenging activity on hydrogen peroxide, DPPH radical and superoxide anion, and also effect on COX-2 and viability on carcinoma cell were all determined by various fractions of cranberry juice. EA fraction was found the main contributor for biological activities of cranberry juice. To further evaluate the effect of cranberry juice on antioxidation and blood lipid regulation in human body. A randomized, placebo control study was designed. Forty subjects were included in this study. Two weeks of run-in period, 10 weeks of administration. Human study showed that 6 weeks of cranberry juice administration greatly increased the antioxidant status, including higher antioxidant capacity, lower thiobarbituric acid reactive substance (TBARS) value, higher ascorbic acid and total thiol contents in blood than those of placebo. In addition, oxidative lag time of LDL was significantly increased from 50.67 to 60.67 (mins). In conclusion, cranberry juice showed great antioxidation, this was probably a prevention of cardiovascular disease. The main contribution was probably mainly from the phenolics of cranberry juice.

並列關鍵字

Cranberry Antioxidation LDL Scavenging radical COX-2

參考文獻


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