蛹蟲草(Cordyceps militaris)又名北蟲草或北冬蟲夏草,與冬蟲夏草同屬於真菌之蟲草屬,其藥理、藥效及病理作用與冬蟲夏草大致相同。目前蛹蟲草已可用人工培育的方式大量生產,且在許多文獻中被證實具有抗腫瘤、調節免疫力和改善疲勞等功效,顯示蛹蟲草可能具有多樣之藥理活性。本實驗研究目的是測試經由熱水萃取的蛹蟲草子實體萃取物(CME)是否具有抗氧化、抗發炎與抗血管新生的能力。在總抗氧化能力測試(trolox equivalent antioxidant capacity assay)中,CME表現出良好的抗氧化能力;而在發炎反應的試驗上,結果顯示CME具有抑制LPS及IFN-γ所活化的巨噬細胞(RAW 264.7)產生一氧化氮(NO)及iNOS基因表現的功能;在血管新生試驗中,首先藉由人類臍帶靜脈內皮細胞(HUVEC)的增生、移動和類血管生成試驗,觀察CME在抑制血管新生上的活性,結果發現CME可抑制HUVEC的增生、移動與類血管形成之功能,其次,在雞胚尿囊絨毛膜的血管新生試驗中,CME也具有抑制血管內皮生長因子(VEGF)刺激雞胚尿囊絨毛膜產生的新生血管的功能。本實驗結果顯示:蛹蟲草熱水萃取物在抗氧化、抗發炎與抗血管新生上確實有良好的效果,此一結果對於未來應用蛹蟲草於保健食品或醫藥用品之開發,可提供一個良好的參考指標。
Cordyceps militaris, a parasitical fungus in moth pupae, is very close to Cordyceps sinensis both in morphology and phylogenesis. It has been reported that Cordyceps militaris and Cordyceps sinensis have similar pharmacokinetics. Recently, it was reported that the artifical cultivation of Cordyceps militaris is successfully developed in industrial large scale. The purpose of this study was to investigate the antioxidant, anti-inflammatory and anti-angiogenic effects of hot water extracts from Cordyceps militaris (CME). In the trolox equivalent antioxidant capacity (TEAC) assay, CME revealed a high level of antioxidant capacity. In anti-inflammatory test, CME inhibited the NO production and iNOS expression in LPS and IFN-γ-stimulated RAW 264.7 macrophage cells. In anti-angiogenic assay, CME inhibited the proliferation, migration and tube-like formation in human umbilical vein endothelial cell (HUVEC). Moreover, CME showed a potent inhibitory activity upon VEGF-induced angiogenesis on the chick embryo chorioallantoic membrane vascularisation (CAM) assay. These functional studies provide a beneficial evidence on the development of health food and drug from Cordyceps militaris.
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