- 學位論文
基質細胞衍生因子-1與血液透析患者的發炎及營養標記之關聯性
Stromal Cell-Derived Factor-1 is Associated with Inflammatory and Nutritional Markers in Hemodialysis Patients
摘要
研究背景：基質細胞衍生因子（stromal cell-derived factor 1，SDF-1）的功能為在腎臟損傷時維持組織新生及再生恆定，然其對血液透析（HD）患者的效應尚未明瞭。 研究方法：本研究於2007年12月收錄152名血液透析患者，並持續追蹤至2012年12月。基質金屬蛋白酵素（matrix metalloproteinase-2/-9，MMP-2/-9）、骨髓過氧化酶（myeloperoxidase，MPO）、骨橋蛋白（osteopontin）及SDF-1利用酵素結合免疫吸附分析法測量；老人營養風險指標（Geriatric Nutritional Risk Index，GNRI）分數之計算方法為利用下列公式修正老年患者的營養風險指標：GNRI = [14.89 x 白蛋白(g/dL)] + [41.7 x（體重/理想體重）]。收錄五十名腎功能正常者作為對照組。 研究結果：對照組受試者平均年齡為40.06 ± 16.54歲，平均血清肌酸酐為0.76 ± 0.17 mg/dl；血液透析患者平均年齡為58.7 ± 14.07歲。相較於對照組，HD組的SDF-1（2946.62 ± 1314.2 pg/ml vs. 2242.99 ± 607.84 pg/ml, p<0.001）與MPO（64.9 ± 24.15 ng/ml vs. 55.39 ± 15.01 pg/ml, p = 0.012）較高，但骨橋蛋白（21.35 ± 41.02 ng/ml vs. 90.46 ± 130.87 ng/ml, p=0.017）、MMP-2（455.89 ± 175.15 ng/ml vs. 517.8 ± 220.86 ng/ml, p=0.077）與MMP-9（334.24 ± 179.18 ng/ml vs. 1165.05 ± 545.07 ng/ml, p<0.001）較低；SDF-1濃度與MMP-2（r = 0.31, p < 0.001）、MMP-9（r = 0.19, p = 0.021）、MPO（r = 0.532, p<0.001）及骨橋蛋白（r = 0.31, p<0.001）呈正相關，其與白蛋白（r = -0.192, p = 0.018）、GNRI分數（r = -0.227, p = 0.007）及標準化蛋白質代謝率（normalized protein catabolic ratio，nPCR）（r = -0.21, p = 0.012）呈負相關。 結論：SDF-1可能影響血液透析患者的發炎及營養狀態，然仍須進一步研究來探討。
並列摘要
Background: Stromal cell-derived factor 1 (SDF-1) functions to maintain homeostatic tissue renewal and regeneration upon renal injury but its effects were not clear on hemodialysis (HD) patients. Methods: In December 2007, 152 HD patients were enrolled and received follow up until December 2012. Matrix metalloproteinase-2/-9 (MMP-2/-9), -myeloperoxidase (MPO), osteopontin and SDF-1 were measured by enzyme linked immunosorbent assay. The Geriatric Nutritional Risk Index (GNRI) score was calculated by modifying the Nutritional Risk Index for elderly patients based on the following formula: GNRI = [14.89 x albumin (g/dL)] + [41.7 x (body weight / ideal body weight)]. Fifty persons with normal renal function were enrolled as a control group. Results: The average age of the control group was 40.06±16.54 years old and the average serum creatinine was 0.76±0.17 mg/dl. The average age of HD patients was 58.7±14.07 years old. As compared with control group, the HD group had higher SDF-1 (2946.62±1314.2 pg/ml vs. 2242.99±607.84 pg/ml, p<0.001) and MPO (64.9±24.15 ng/ml vs. 55.39±15.01 pg/ml, p=0.012), but lower osteopontin (21.35±41.02 ng/ml vs. 90.46±130.87 ng/ml, p=0.017), MMP-2 (455.89±175.15 ng/ml vs. 517.8±220.86 ng/ml, p=0.077) and MMP-9 (334.24±179.18 ng/ml vs. 1165.05± 545.07 ng/ml, p<0.001 ). SDF-1 level indicated-positive correlations with MMP-2 (r = 0.31, p < 0.001), MMP-9 (r = 0.19, p = 0.021), MPO (r = 0.532, p<0.001) and oeteopontin (r=0.31, p<0.001), and negative correlations with albumin (r=-0.192, p=0.018), GNRI score (r=-0.227, p=0.007), and normalized protein catabolic ratio (r =-0.21, p = 0.012). Conclusions: SDF-1 may have a role in influencing the inflammation and nutrition status of HD patients but further studies are necessary to investigate this possibility.
參考文獻
延伸閱讀
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