加味四物湯抗氧化性以及對肝臟與皮膚之生理研究

Study of modified Si Wu Tang on antioxidation and physiological effect on liver and skin

吳英華(Ying- Hua Wu)

指導教授 ： 王進崑 ； 陳肅霖

中山醫學大學/健康管理學院/營養學研究所/碩士(2009年)

https://doi.org/10.6834/CSMU.2009.00131

摘要

中文摘要
四物湯是傳統的中藥之ㄧ，是由當歸、川芎、芍藥和熟地黃四種中藥材組成，在台灣民眾的觀念裏，四物湯是被用來治療婦女病癥。四物湯含有多種有機酸、胺基酸、多醣類、維生素、礦物質及酚類化合物等成分，四物湯被發現具有抗氧化、抗衰老、清除自由基等功效。本研究目的在於了解服用四物湯是否具有效抗氧化性及對肝臟與皮膚生理上變化，包括改善人體血漿抗氧化能力、增加抗氧化酵素活性、護肝及皮膚改善之功效。本試驗共計有55位健康之受測者(男性10人，女性45人)全程參與，分為兩組同步進行，本試驗採隨機雙盲、安慰劑對照暨交叉設計。實驗期間共計十三個月，一組在試驗前半年服用安慰劑，後半年服用靈芝，中間的一個月為wash out period；另一組則是先服用四物湯再服用安慰劑。試驗期間第零、三、六、十及十三個月，需作測量體位、收集血液檢體，且於試驗中第零、六、及十三個月進行腹部超音波檢測和皮膚檢測。
結果顯示，每人每月連續服用六天四物湯，每次飲用120mL(相當於攝取11.71公克多醣體、0.5公克類黃酮化合物及68.4毫克酚類化合物)，連續服用六個月後，可以顯著增加受測者體內總抗氧化能力及顯著降低脂質之過氧化反應，顯著提高紅血球中超氧歧化酶、觸酶及麩胱甘肽還原酶等抗氧化酵素之活性。
此外，由腹部超音波檢測的結果也顯示，服用四物湯可改善受測者脂肪肝、膽結石和輕微脾腫大的情況。皮膚檢測的結果可知，服用四物湯後可減少皮膚水分散失，增加皮膚紅潤度及改善皮膚紋理的效果。

關鍵字

四物湯 ； 多醣體 ； 類黃酮化合物 ； 酚類化合物 ； 抗氧化性 ； 肝功能 ； 皮膚檢測

並列摘要

Abstract

Si Wu Tang is one traditional Chinese medicine, Si Wu Tang is

an old formula containing four herbs, radix Angelicae sinensis (Dang

Guay) , rhizoma Ligustici chuanxiong (Tsuan Chyong), radix Paeoniae

alba (Bai Sau ), and radix Rehmanniae praeparata (Soe Dee Huang),

It has been widely used to relieve menstrual discomfort in Taiwan.

Si Wu contains a lot of organic acids、amino acids、polysaccharides、

vitamins、minerals and phenolic compounds .Many animal studies

indicate that Si Wu Tang has antioxidation and anti-aging effect. This

study was modified Si Wu Tang on anti-oxidation and physiological

effect on liver and skin, including anti-oxidation ability of plasma,

erythrocyte enzyme activities, liver function protection and the

improvement on skin condition. This was a randomized, placebo

controlled, double blind and cross over design.55 subjects (10 males and

45 females)were completed involved in this study. The total

experimental period was thirteen months. One group of subjects took

placebo in the first six months and Si Wu Tang in the next six months,

one month between this two periods was wash out period. The other

group of subjects took Si Wu Tang in the first six months and placebo in

the next six months. Anthropometric measure-ements and blood

collection were performed at the initial,3rd,6th,10th,13th month of the

testing period. Abdominal ultrasound and skin examination were

performed at the initial,6th,13th months. Each subject drank 120mL Si Wu

Tang (11.71g polysaccharides, 0.5g flavonoid compounds and 68.4mg

phenolic compounds) everyday for six days per month.Results showed

that six months continuous administration of Si Wu Tang significantly

increased total antioxidant capacity and reduced TBARS contents in

plasma. The antioxidant enzyme activity of superoxide dismutase(SOD),

catalase and glutathione reductase (GSH Rd) in erythrocytes was also

increased.

In addition,the abdominal ultrasound examination showed

improvement on fatty liver, gall stone and mild splenomegaly. Increasing

skin erythema, decreasing skin surface water loss and reducing skin

wrinkles were found by the administration of Si Wu Tang.

並列關鍵字

Si Wu Tang ； polysaccharides ； flavonoid compounds ； phenolic compounds ； antioxidation ； liver function ； skin examination

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