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  • 學位論文

洛神花花青素促進人類腸癌LoVo細胞凋亡之作用

Hibiscus anthocyanin induced apoptosis in human colon cancer cell

指導教授 : 王朝鐘
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摘要


癌症預防已為一世界潮流,因此,尋找一有效暨安全化學預防物質為一重要工作。花青素(Anthocyanins)及多酚(Polyphenol)廣存於蔬菜、水果等植物中,已有許多報告證明洛神花花青素具抗氧化活性,降低血脂肪,預防動脈粥狀硬化及防癌等療效,近年來本實驗室致力於抗癌的研究顯示此類Hibiscus anthocyanins(HAs)及Hibiscus polyphenol(HPE)會促進胃癌,肝癌,血癌,乳癌,等細胞凋謝死亡,因此,本研究擬探討HAs對於人類腸癌LoVo細胞之抗癌作用及其作用之分子機制。 中草藥已沿用於各項疾病多年,其中亦包含針對癌症的治療。另一方面,近年來的研究顯示癌細胞因無法正常凋亡而生長蔓延,因此探討其細胞凋亡機轉成為研究癌症治療當中一項重要的課題。 本研究利用細胞毒性試驗、MTT assay、DAPI染色、FACS、粒腺體膜電位染色等方法觀察,初步的結果顯示洛神花花青素確實會誘發人類腸癌細胞凋亡的情形。其作用的機轉可能為抑制NF-kB及I-kB的表現,而增加Fas-L與Fas的表現量活化caspase-8,且Bcl-2蛋白族群的參與,促進粒腺體cytochrome C的釋放,進而開啟一連串caspase cascade訊息傳遞的活化而導致細胞凋亡路徑。 洛神花花青素可促進腸癌細胞的凋謝死亡以及預防或延緩腸癌發展進程。根據本篇的結果洛神花花青素其具有抗腫瘤的療效,未來可應用於化學預防上成為發展新藥。

關鍵字

花青素 細胞凋亡

並列摘要


It’s a popular topic of discussing how to prevent the formation of cancer. The development of new drugs are both safe and effective will be important for the future of cancer chemoprevention. Many studies demonstrate that Anthocyanins and Polyphenol widely in many plants have the effects of antioxidation, hypolipidemic, anti-atherosclerotic and anti-carcinogenesis, such as the Anthocyanins in the Hibiscus sabdariffa L. Our previous reports demonstrated that anthocyanins extracted from Hibiscus sabdariffa Linne, Hibiscus anthocyanins (HAs) and Hibiscus polyphenol(HPE)induced apoptosis effect on human cancer cells. In this study, we explored the anti-cancer effect and the particularly mechanism of HAs on human colon cancer cell. The Chinese herbal medicine has already been continued to use for many years at many disease, has also included directing against the treatment of the cancer. On the other hand, the research in recent years shows the cancer cell grows and spreads because of being unable to wither and die normally. It’s an important subject to detect the cancer cells with specially mechanism in cancer therapy. We used MTT assay, DAPI staining, flow cytometery, mitochondria membrane potential analysis and western blot to detect and found that HAs treatment markedly induced apoptosis in LoVo cells in a dose- and time-dependent manner. The results also reveal inhibited NF-kB, induced by members of the death receptor such as Fas/FasL that leads to the formation of pro-caspase-8, increased cytochrome C release, and expression of Bcl-2 family protein via mitochondrial death pathway. Followed, the mechanism of apoptosis is associated with activation of the caspase cascade in the HAs-treated LoVo cells. We suggested that HAs could promote colon cancer cell apoptosis and prevent or delay cancer cell progression. According to these results, HAs has a potential in anticancer effect that could be developed as chemopreventive materials.

並列關鍵字

HAs apoptosis

參考文獻


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被引用紀錄


姚芳儀(2011)。探討洛神花青素抗乳癌細胞之作用及其分子機制〔碩士論文,中山醫學大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0003-2308201114270500

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