- 學位論文
生醫材料對細胞外基質與行為影響之研究
Studies of Biomaterial effect to the Extracellular Matrix Expression and Cellular Behavior
摘要
周邊神經受損時，主要的治療方式為自體神經移植。神經導管是替代方法之一。以Chitosan為生醫材料製作的導管，搭配Schwann cell共同培養的生物活性導管，所產生的神經生長促進因子，laminin及collagen 4明顯增加，顯示該導管對於受損的周邊神經，應具有促進再生修復的潛力。此結果也顯示了，立體構型的支架，除了可提供充裕的生長空間，對於細胞產物的增加也具有相當的影響。 另一方面，在細胞與培養表面接觸時，表面構型的立體結構，會影響細胞貼附、展開及移動的能力。實驗中以平面及不同圓柱密度的表面構型，分析細胞完成早期貼附至移動的動態變化。結果顯示圓柱密度對於細胞型態，及移動能力具有直接的影響；對於細胞膜及核酸物質的分佈，也與表面圓柱構型的疏密具有明顯關係。 上述實驗，說明了生醫材料的表面構型，不僅影響細胞型態，對於次細胞 ( sub-cellular ) 階層的蛋白質表現，以及DNA、RNA的分佈調控，也具有相當的影響。
並列摘要
1. Chitosan scaffolds effect extracellular matrix expression Cellular adhesiveness to biomaterial is one of the important properties to the success of tissue engineering. The cell-biomaterial interactions involve close cooperation of adhesion proteins, the plasma membrane, and cytoskeletons in order to form focal adhesions during the process of anchoring. Dynamic development of the plasma membrane in the process reflects the cellular biocompatibility and motility. The process of cell attachment beginning from seeding, contact, attachment, spreading has not been investigated. In this study, we monitored the whole process of cells attaching to the substrate surface by time-lapse confocal microscopy. We observed that the surface configuration of the substratum effects plasma membrane expansion and genomic materials distribution. In contrast to the cells grown on the plate, the cells attached on pillars are with rounded nuclei and with prominent lamellipodia spreading out. Membrane expansion is involved in dynamic development of the plasma membrane and lamellipodia formation for attachment, migration or proliferation and reflects the cellular physiology status of the cells. This study provides a platform for investigation of cell behavior and dynamic development of subcellular structures regarding cell-biomaterial interactions 2. Cellular behavior and substrate configuration Chitosan is a choice material for scaffolds in regenerative medicine. One of the applications is to bridge the damaged peripheral nerves. Previous studies showed that combination of chitosan conduits and cultured Schwann cells could increase the opportunity for re-connection of broken nerves. It has also been known that Schwann cells can produce the ECM components which are critical for nerve regeneration. In this study, we used the rat Schwann cells ( RSCs ) grown on porous chitosan scaffolds for quantitative analysis of ECM protein expression. The RSCs grown on chitosan scaffolds secreted higher amount of laminin and collagen 4 than those grown on the plan. The increased laminin and collagen 4 produced by Schwann cells could create a preferable condition for stimulating peripheral nerve regeneration.
參考文獻
延伸閱讀
- Li, Y. C. (2013). 生醫材料與培養基成份對神經幹細胞/前驅細胞行為之探討 [doctoral dissertation, National Taiwan University]. Airiti Library. https://doi.org/10.6342/NTU.2013.00934
- 薛文景、王馨偵（2007）。生醫材料表面改質對細胞培養之研究。鑛冶：中國鑛冶工程學會會刊，51(2)，139-142。https://doi.org/10.30069/MM.200706_51(2).0012
- 陳奕叡（2003）。探討不同藥物對基質金屬蛋白酵素活化之影響及其在人類初級與細胞株內皮細胞之差異〔碩士論文，臺北醫學大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0007-1704200714513291
- Wang, Y. H. (2019). Development of innovative biomaterials for slow proliferating human tissues [doctoral dissertation, National Taiwan University]. Airiti Library. https://doi.org/10.6342/NTU201902344
- Chen, P. Y., Lin, A. Y. M., Lin, Y. S., Meyers, M. A., & McKittrick, J. (2010). Structure, Function and Mechanical Properties of Selected Biological Materials. 安泰醫護雜誌, 16(3), 135-166. https://doi.org/10.7078/TSMHMNJN.201009.0135