由近幾年來所發表的文章可以觀察到,科學家對於長波長螢光劑運用於生理活體即時追蹤分析深感興趣。螢光分子螢光波長位於近紅外光區域(λem> 600nm)的最大優點即為能讓光滲透到活體組織內,不受活體自發現螢光干擾,增加靈敏性有效提升活體的即時影像監控。因此開發新型近紅外光螢光分子將有助於生物影像研究。我們利用Benzimidazol iminocoumarin 當起始物模版合成一系列的衍生物且探討他們的光學性質研究。我們所合成的一系列Benzimidazol iminocoumarin衍生物具有大於600nm的螢光釋放波長,符合長波長螢光劑的螢光釋放波長範圍;另外,這些化合物的螢光量子效率約為10~90%。
There are growing interests in employing near-IR fluorophores in various analytical applications such as immunoassay, liquid chromatography(LC), capillary elecrophoresis(CE) and especially the real time in vivo biological imaging. Photo penetration into living tissue is highly dependent on the absorption and scattering properties of tissue components. The near-infrared region of the spectrum offers certain advantages for photon penetration, and both organic and inorganic fluorescence contrast agents are now available for chemical conjugation to targeting molecules. Furthermore, employing NIR fluorophores in biological imaging significantly reduced the back ground signal due to the lowest autoabsorption and atuofluorescence of biolmolecule in the long-wavelength region of spectrum. We employed benzimidazole iminocoumarin as the synthetic synthon to synthesized a series of derivations and studied their photophysical properties. The benznmidazol iminocoumarine derivatives synthesized rein exhibited emissions spectra in the red or NIR region. The fluorescence quantum yield ranges from 10 to 90%.
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