應用磁性材料檢測沙門氏菌的抗藥性

Utilization of magnetism material for detecting antibiotic resistance of Salmonella

謝承志(Cheng-Chih Hsieh)

指導教授 ： 黃榮堂

國立臺北科技大學/機電學院/機電整合研究所/碩士(2012年)

摘要

全世界包含台灣，沙門氏菌是幼兒腸道炎中常見的病菌；而現行檢測沙門氏菌濃度數量及其抗藥性需耗費三至五天的時間。若能早期檢測出檢體是否具有抗藥性，不但能夠幫助醫師選擇適當的抗生素，也可以避免抗生素濫用的情形。本研究之目的為發展簡單、快速之檢測方式好取代早期檢測細菌及其抗藥性之方式，利用微機電製程技術製作用於實驗之微粒檢測的裝置晶片。本研究使用新式複合材料－奈米金磁珠，使其接合與沙門氏菌具專一性之抗體。並利用微機電製程技術，製作含有鎳金屬之平行梳狀電極。運用磁力的作用，有效的從糞便中分離出沙門氏菌，達成純化的效果。且利用磁力使鎳金屬具有磁性並吸引已接上沙門氏菌之奈米金磁珠，再以阻抗分析儀進行組抗量測。藉由量測結果可以判斷該檢體沙門氏菌的濃度，而後可加入抗生素進行其抗藥性檢測。加入抗生素後做養菌動作並量測其阻抗值，藉由阻抗值的變化來判斷抗藥性與否。

關鍵字

微機電製程技術 ； 奈米金磁珠 ； 梳狀電極 ； 抗藥性

並列摘要

Asia, including Taiwan, Salmonella is one of the common enteric pathogenic bacteria in children all over the world including Taiwan. Detecting of the concentration and resistance of Salmonella takes three to five days, which is time-consuming. Early detection of antibiotic resistant can not only help doctors to choose appropriate antibiotics, but also avoid the abuse of antibiotics. The purpose of this study is to develop a simple and rapid detection method for early detection of bacteria and antibiotic resistance which can replace the traditional way, using Micro Electro Mechanical Systems process technology for production of the bio-chip particle detection. In this study, we use the new composite materials - nano Au/Fe3O4 particles for bonding the specificity of the antibody with Salmonella. Using of MEMS process technology, we produced the nickel parallel interdigitated electrodes. Using magnetic force, we can effectively isolate Salmonella from stools to reach the effect of purification. Utilization of magnet provides the nickel electrodes with magnetic which attract the nano Au/Fe3O4 particles connected to the Salmonella and then the impedance can be measured on the bio-chip. According to the results, we can measure the concentration of Salmonella in the samples, and add antibiotics to test its antibiotic resistance. Following addition of antibiotics, culture of bacteria and impedance measurement of the antibiotic-contained bacterial solution, the antibiotic resistance of the selected bacteria can be determined according to the alterations of impedance.

並列關鍵字

MENS ； nano-Au/Fe3O4 particles ； interdigitated electrode ； antibiotic resistance

參考文獻

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