RGD改質後之海藻酸鈉水凝膠對包埋在內的纖維母細胞生長與分化之影響

In Vitro Studies of Fibroblasts Embedded in Hydrogelic Fibers Made of RGD-modified Alginate

李彥瑩(Yen-Ying Li)

指導教授 ： 林忻怡

國立臺北科技大學/工程學院/生物科技研究所/碩士(2014年)

https://doi.org/10.6841/NTUT.2014.00548

摘要

在組織工程中，支架通常是水膠做成的是具有多孔性、可解性，海藻酸鈉是無毒、溶於水的天然高分子材料，所以可以與細胞混合，讓細胞較均勻的分布，RGD胜肽會與某些細胞表面受體結合促進細胞貼附、生長，為了讓細胞在水凝膠纖維中生長能夠生長得更好，所以使用RGD胜肽改質海藻酸鈉，再用快速原型機製作立體結構的水凝膠纖維，比較在改質前後海藻酸鈉水凝膠內細胞生長的差異性。
研究發現，在DNA定量中，可以看從第四天到第十四天有改質過的海藻酸鈉中DNA含量比沒改質的高；由細胞存活螢光染色觀察細胞存活率，可以知道從第一天到第十四天兩組支架內的大多數的細胞接存活下來，在第十四天RGD改質過的海藻酸鈉有看到比較細長的形狀，在細胞貼附實驗中，發現細胞在第二天開始呈現梭狀，而且RGD-alginate海藻酸鈉薄膜上的呈現梭狀的細胞明顯比較多。
結果顯示，我們有成功接枝RGD胜肽到海藻酸鈉上，提供更適當的條件讓細胞生長。在未來，希望RGD海藻酸鈉可以運用在傷口敷料上，讓細胞可以再更適合的環境與條件，幫助傷口癒合。

關鍵字

海藻酸鈉 ； RGD胜肽纖維母細胞

並列摘要

In tissue engineering, it is important that scaffolds require porosity, biocompatibility and degradability. Alginate is a natural material to fabricate scaffold. RGD peptide that can promote cell adhesion, cell growth and help wound healing is combined with some surface receptors of cell.
For improve cell distribution, we mix the alginate and cells together. We modified alginate with RGD and embedded cells in hydrogel fibers made of RGD-alginate scaffold using rapid prototyping. Cell morphology and proliferation inside the RGD-alginate hydrogel were examined and compared to cells embedded in alginate.
In this study, we knew the DNA quantification of the RGD-alginate is higher than alginate . Live/dead stain showed all cells are alive in two scaffolds. HE stain appeared the cell’s morphology no significant difference in two kinds of scaffolds. Results show that RGD-alginate scaffold is more stable for cell to growth.

並列關鍵字

alginate ； RGD peptide ； fibroblast ； rapid protoyping

參考文獻

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