超音波微氣泡對比劑結合凝膠之聲學特性與應用

超聲波微泡對比劑（MBs），主要是注入血管內用於診斷或治療。在我們先前的研究指出，將微氣泡對比劑混合在液體中有效的增加經皮給藥的吸收。本研究目的改變介質為凝膠微氣泡對比劑，來驗證其提高外用經皮給藥的穿透效果的可行性。本實驗使用美白成分維他命C磷酸鎂鹽(MAP)來驗證體外及體內實驗的可行性。在體內實驗部分，將動物隨機分成五組: (1) 控制組 (C) (2) 塗抹MAP組 (M) (3) 超音波結合MAP組 (UM) (4) 超音波結合0.1%的凝膠混合微氣泡對比劑組 (UMB1) (5)超音波結合0.15%的凝膠混合微氣泡對比劑組 (UMB2)。根據結果顯示，仿體實驗及豬皮實驗中，在0.1%的Agarose凝膠混合微氣泡對比劑分別提升40%及195%的穿透深度，而在0.15%的Agarose凝膠混合微氣泡對比劑分別提升48%及205%的穿透深度。小鼠動物實驗美白效果 (亮度值 (L*) ) 顯示，第一週UMB1與UMB2分別增加了30.8%與30.9%，第二週分別增加49%及57%，第三週開始趨於穩定分別增加59%及63%，第四週趨於穩定 (63%及%70)，在四週的治療期間，我們的實驗結果顯示超音波結合凝膠混合微氣泡對比劑可以增加皮膚通透度，以加強MPA滲透抑制黑色素形成，且不傷小鼠皮膚。

關鍵字

超音波微氣泡對比劑；維他命C磷酸鎂鹽； Agarose瓊質凝膠；凝膠微氣泡；經皮給藥；小鼠美白實驗；穴蝕效應

並列摘要

Ultrasound contrast agent, microbubbles (MBs), is mainly injected to the blood pool for diagnostic and therapeutic applications. In our previous study, the feasibility and effects of the MBs in the liquid as the penetration enhancers for transdermal delivery were firstly demonstrated. In this study, the MBs in gel medium for transdermal delivery was demonstrated to enhance the possibility of external used. The penetration of magnesium ascorbyl phosphate (MAP) on skin was enhanced by using ultrasound energy and MBs in gel medium either for in vitro or for in vivo experiments. In vivo experiment parameters were randomly divided into five groups: (1)control (C); (2) only penetrating MAP (M); (3) ultrasound combines with penetrating MAP (UM) (4) ultrasound combines with MBs contrast agent in 0.1% agarose solution and penetrating MAP (UMB1); (5) ultrasound combines with MBs in 0.15% agarose solution and penetrating MAP (UMB2). According to the results, the penetration depth of agarose phantom and pig skin increase 40% and 195% in MBs contrast agent in 0.1% agarose solution group, increase 48% and 205% in MBs contrast agent in 0.15% agarose solution group. The whitening effect (luminosity index (L*)) of mice skin in UMB1 and UMB2 groups have significantly increase 30.8% and 30.9% in one week, 49% and 57% in two weeks, 59% and 63% in three weeks and tends towards stability in four weeks (63% and 70%) in C57BL/6J mice over a 4-week experimental period. Our results investigated that the treatments of ultrasound and MBs in gel medium also can increase skin permeability, enhance MAP delivery to inhibit melanogenesis and not damage the skin in mice.

並列關鍵字

Ultrasound contrast agent ； Microbubbles ； Magnesium ascorbyl phosphate ； Agarose gel ； Transdermal drug delivery ； In vivo ； Cavitation

參考文獻

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[4] 郭育辰，振幅調變啾聲影像於超音波對比劑偵測，碩士論文，國立清華大學生醫工程與環境科學系，新竹，

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超音波微氣泡對比劑結合凝膠之聲學特性與應用

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