cAMP依賴性的蛋白激酶對過度糖化最終產物誘導 RAW264.7吞噬細胞iNOS表現和NO產生的調控

Involvement of cAMP Dependent Protein Kinase in BSA- AGEs-induced iNOS Expression and Nitric Oxide Production in RAW 264.7 Macrophages

陳健民(ChienMing CHEN)

指導教授 ： 李宏謨

臺北醫學大學/醫學院/醫學檢驗生物技術學研究所/碩士(2001年)

摘要

過度糖化最終產物(AGEs)是糖與蛋白質經非酵素反應及長時間修飾後的產物，它會影響結構和功能改變，並與高齡及長期糖尿病人常見的併發症，如退行性神經變化及糖尿病之腎及血管併發症有關。以前我們曾証明AGEs誘導C6 Glioma cells之iNOS表現可經由p38 MAPK路徑。本實驗主要探討cAMP依賴性蛋白激酶對於AGEs誘導RAW 264.7吞噬細胞 (RAW 264.7 細胞) iNOS表現和NO產生的調控作用。AGEs誘導RAW 264.7 細胞之iNOS表現和NO的產生隨著時間與劑量而增加，這種由AGEs誘導RAW 264.7 細胞產生NO和iNOS的表現可被蛋白激酶A (PKA)的抑制劑KT 5720 (1 ?M) 和H8 (10 ?M) 所抑制。PKA直接活化物dibutyryl cAMP (Bt2 cAMP) 於不同濃度刺激 RAW 264.7 細胞，結果隨著濃度增加而提升NO的產生和iNOS的表現。AGEs刺激細胞內cAMP的產生是隨著時間增長而持續增加。為了証明AGEs誘導NO的產生和iNOS的表現不是因LPS的污染，本實驗以不同濃度之LPS抑制劑 polymyxin B加入含AGEs之RAW 264.7 細胞，結果發現AGEs誘導NO的產生不會被 polymyxin B所影響，證實AGEs的作用與LPS不同。 AGEs可於RAW 264.7 細胞活化p38 MAPK的產生，此效果可被KT 5720 (1 ?M)，H8 (10 ?M) 和SB 203580 (10 ?M) 所抑制。因此由實驗推測AGEs可造成cAMP增加，轉而活化PKA，結果使p38 MAPK活化最後造成iNOS表現與NO的產生。

關鍵字

過度糖化最終產物 ； 一氧化氮 ； 吞噬細胞

並列摘要

Advanced glycosylation end products (AGEs) have been implicated in the structural and functional alterations of proteins that occur during aging and long-term diabetes. Previously, we have demonstrated that p38 MAPK is involved in the AGEs-induced iNOS expression in C6 glioma cells. In the present study, roles of cAMP dependent protein kinase in AGEs-induced iNOS expression in RAW 264.7 macrophages were investigated. AGEs caused a dose- and time-dependent increase of nitric oxide (NO) accumulation and iNOS expression in murine RAW 264.7 macrophages. The AGEs-simulated NO production and iNOS expression was dose-dependently inhibited by the PKA inhibitor, KT 5720 (1 ?M) and H8 (10 ?M). Consistently, treatment of RAW264.7 macrophages with dibutyryl cAMP results in concentration-dependent NO release and iNOS induction. On the other hand, AGEs-stimulated cAMP production in RAW 264.7 cells was seen in 1 h and persisted for at least 24 h. The NO production was not affected by polymyxin　B, a lipopolysaccharide (LPS) inhibitor, suggesting the NO production is not due to LPS contamination in the BSA-AGEs (AGEs) preparation. AGEs actived p38 MAP kinase in RAW 264.7 cells and this effect was blocked by KT 5720 (1 ?M), H8 (10 ?M) and SB 203580 (10 ?M). The p38 MAPK inhibitor (SB 203580) did not inhibit AGEs-induced cAMP accumulation. In conclusion, our data suggest that AGEs may increase intracellular cAMP, which in turn activates PKA and results in p38 MAPK activation, iNOS induction and NO production in RAW 264.7 macrophages.

並列關鍵字

Advanced glycosylation end-products ； Nitric oxide ； RAW 264.7 macrophages

參考文獻

Brett, J., Schmidt, A. M., Yan, S. D., Zou, Y. S., Weidman, E., Pinsky, D., Nowygrod, R., Neeper, M., Przysiecki, C., and Shaw, A. (1993) Survey of the distribution of a newly characterized receptor for advanced glycation end products in tissues. Am. J. Pathol. 143, 1699-1712

Amore, A., Cirina, P., Mitola, S., Peruzzi, L., Gianolio, B., Rabbone, I., Sacchetti, C., Cerutti, F., Grillo, C., and Coppo, R. (1997) Nonenzymatically glycated albumin (Amadori adducts) enhances nitric oxide synthase activity and gene expression in endothelial cells. Kidney Int. 51, 27-35

Browning, D. D., McShane, M. P., Marty, C., and Ye, R. D. (2000) Nitric oxide activation of p38 mitogen-activated protein kinase in 293T fibroblast requires cGMP-dependent protein kinase. J. Biol. Chem. 275, 2811-2816

Brownlee, M. (1991) Glycosylation products as toxic mediators of diabetic complications. Ann. Rev. Med. 42, 159-166

Chang, C. I., Zoghi, B., Liao, J. C., and Kuo, L. (2000 ) The involvement of tyrosine kinase, cyclic AMP/protein kinase A, and p38 mitogen-activeted protein kinase in IL-13-mediated arginase I induction in macrophages: its implications in IL- 13-inhibited nitric oxide production. J. Immunol. 165, 2134- 2141