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  • 學位論文

蘭陽盆地居民尿液硒含量和無機砷甲基化代謝能力之相關性研究

The Relationship between Urine Selenium Levels and Inorganic Arsenic Methylation Capability among Residents of Lanyang Basin in Taiwan

指導教授 : 薛玉梅
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摘要


本研究目的探討尚在飲用地下水的蘭陽盆地居民,暴露於高濃度的無機砷下,其無機砷代謝能力之分布。硒為人體必須金屬,亦具抗氧化作用。動物、體外試驗及流行病學研究中發現硒對無機砷有拮抗作用。因此本研究同時探討蘭陽盆地的居民其血清及尿液中總硒量與無機砷甲基化代謝能力之間的相關性。 民國八十年設籍於當地40歲以上之居民12000人中參與問卷訪視者共計8102人,依飲用地下水砷暴露濃度分組隨機選取317人為研究對象。收集研究對象所飲用之井水及尿液儲存於-200 C,血液儲存於-700 C冰庫中。以感應耦合電漿-原子發射光譜分析法測定井水砷濃度,以石墨爐式原子吸收光譜儀測定血清中總硒量以及尿液中總硒量與總砷量,利用高效能液相層析儀連結氫化器原子吸收光譜儀測定尿液中的無機砷甲基化代謝物種,並以統計軟體進行統計分析。 結果發現性別、抽煙或飲酒與否與慢性砷暴露指標(飲用含砷井水年數,飲用井水含砷濃度,累積砷暴露量)均沒有顯著差異。男性的單甲基砷酸百分比及總砷量比女性顯著偏高而雙甲基砷酸百分比卻比女性顯著偏低。未飲酒者其無機砷百分比較飲酒者低而雙甲基砷酸百分比較飲酒者偏高。年齡小於51歲者其血清硒濃度比58至64者者顯著偏高。男性的尿液硒及砷濃度比女性偏高,未抽菸者尿液硒及砷濃度比抽菸者為低。在調整年齡與性別後,井水含砷濃度越高者則總砷量及尿液砷濃度越高;累積砷暴露量越高則總砷量越高。在調整年齡與性別及其他危險因子後,血清維生素E越高則尿液砷濃度與總砷量越高;無機砷百分比隨尿液硒濃度增加而顯著減少,雙甲基砷酸百分比隨尿液硒濃度增加而顯著增加。尿液硒似乎會使砷甲基化代謝能力增加,此須進一步探討。

關鍵字

微量營養素 甲基化

並列摘要


The objective of this study was to discuss the distribution of the inorganic arsenic metabolism capabilities among residents of Lanyang Basin who drank underground water and exposed to high concentrations of inorganic arsenic. Selenium is a trace essential elements that is important in providing protection against oxidative damage. It was observed selenium produced antagonism against inorganic arsenic at animals, in vitro experiments and epidemiology studies. Therefore, we also discussed the relationship between total selenium levels in serum and urinary arsenic methylation capabilities among the residents in Lanyang Basin. At 1991, 8,102 residents were participated questionnaire interview. According to the arsenic concentration of their drinking water, they were classified into two groups(less or equal to 50 ug/L and over ug/L); from each group, totally 317 persons were randomly sampled. Their drinking well water, urine, and blood were collected and stored in refrigerators at the temperature of -20oC and —70oC respectively. The concentration of arsenic in well water, the total selenium levels in serum, total selenium and arsenic levels in urine, and inorganic arsenic methylation species in urine were determined by inductively coupled plasma atomic emission spectroscopy, graphite furnace atomic absorption spectrometry, and high-performance liquid chromatography with hydride generation atomic absorption spectrometry respectively. The results were analyzed by statistical software. It turned out no differences in chronic arsenic exposure index (the number of years of drinking arsenic well water, the concentration of arsenic of well water, cumulative arsenic exposure) among opposite genders, cigarette smoking or not, and alcohol drinking or not. The percentage of monomethylarsonic acid and the total arsenic levels in male were significantly higher than those in female, but the percentage of dimethylarsinic acid in male were significantly lower than those in female. The percentages of inorganic arsenic in non-alcohol drinkers were lower than those in alcohol drinkers, but the percentage of dimethylarsinic acid in non-alcohol drinkers were higher than those in alcohol drinkers. The concentration of selenium in serum of the residents below 51 years old were significantly higher than those of the residents between 58 and 64 years old. The concentrations of arsenic and selenium in urine in male and smoker were higher than those in female and non-smokers respectively. After adjusting the age and gender, the higher the concentration of arsenic in well water, the higher the total arsenic levels and the concentration of the arsenic in urine; the higher the cumulative arsenic exposure in drinking water, the higher the total arsenic levels in urine. After adjusting the age, gender, and other risk factors, the higher the concentration of Vitamin E in serum, the higher the concentration of the arsenic and total arsenic levels in urine. The percentage of inorganic arsenic significantly decreased while the concentration of selenium in urine increased; the percentage of dimethylarsinate significantly increased while the concentration of selenium in urine increased. It seemed the selenium in urine would increase the metabolism of methylation of arsenic, this needed further investigation.

並列關鍵字

arsenic selenium micronutrant methylation

參考文獻


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被引用紀錄


簡郁鍾(2006)。砷暴露途徑及其與居民尿液砷物種之探討〔碩士論文,中山醫學大學〕。華藝線上圖書館。https://doi.org/10.6834%2fCSMU.2006.00057

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