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  • 學位論文

beta-chitin 作為生物感測器之應用及其相關研究

Application of beta-chitin for biosensor matrix use

指導教授 : 曾厚
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摘要


殼糖是一種身體可吸收性材料,具有生體可相容性及生體可降解性,並且經由一些體內的酵素分解產生中間產物具有免疫賦活性。依造殼糖的結構分為三種,其中a-及b-兩種最為人所知。以往以a-chitin為對象,本研究利用squid bone經由脫鈣脫蛋白的過程精製b-chitin。精製時的濃度、溫度越高,會因為主鏈斷鍵以及分子結構改變造成分子量降低。利用5 % LiCl / DMAC製成殼糖溶液並紡絲成膜,以4 %之殼糖溶液所製成之成形物張力強度最強。在鹼性環境下,利用高溫將殼糖轉變為聚葡萄胺醣,可應用在組織工程學上,作為人工材料使用。利用X-ray繞射儀,可依結晶度比較兩種Type之差異。細胞毒性測試,利用其他材料與殼糖膜相比較,殼糖膜之O.D值分別為115 %及104 %,與其他材料相比,有高度的免疫相容性,適合應用在動物體。材料熱性質測試,比較殼糖CM化前後之差異,CM化殼糖在100°C、 217°C左右產生明顯波峰,可依此特性與未CM化殼糖相比較。FT-IR光譜判定,精製殼糖在IR光譜上產生其特徵吸收峰;CM化之殼糖膜,利用FT-IR之ATR照射,可觀察1700cm-1有-CO官能基產生。經由酵素固定過程,製成酵素殼糖膜作為生物感測器之基質,且進行活性測試,利用分光光度計,可測得1cm2之殼糖膜表面,殘存酵素約0.2 — 0.3 units。未來並希望酵素殼糖膜,在生物感測器有更好的發展。

並列摘要


Chitin, one of the most abundant polymers in Nature, is a biocompatible and biodegrable polymer. In the body, chitin can be digest through by enzyme and is a low immunogenetic poly-saccharide. Chitin can be separated in three types by its structure, with a-and b- types well known. To investigate a-chitin used, in this proposed research, we will apply the technique of wet spinning or casting to develop a facile type of chitin fiber and membrane from the chitin dope, which prepared with high molecular weight shrimp-resource chitin dissolved in metal salt / amine-containing solvent. At highly temperature and concentration, have low molecular weight .The stress is highly at 4% chitin solution. The chitosan scaffolds derived from chitin in the strong base and high temperature bs used for tissue engineering use. In the X-ray diffraction pattern of chitin, some differences were observed between a- and b- types. In the MTT assay, we can find chitin is biocompatible and bioabsorbable. In the thermal analysis, rH of carboxymethylchitin found at temperature 217°C different from other types. We also find the characteric absorbant peak by FT-IR . After enzyme immobolization of chitin membranes, found the residue activity about 0.2-0.3 units at 1 cm2 membrane by the spectrumeter. We hope that the uses of carboxymethylchitin for enzyme immoblization, in the future, can have a good application at the biosensor.

參考文獻


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被引用紀錄


林玫秀(2001)。靈芝子實體殘渣衍生物的抗菌活性之研究〔碩士論文,臺北醫學大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0007-1704200714342496

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