高分子水溶性幾丁聚醣對免疫反應調節之研究

The effects of high molecular weight water soluble chitosan on immune response by in vivo study

董映志(Ying-Chih Tung)

指導教授 ： 謝榮鴻 博士

臺北醫學大學/公共衛生暨營養學院/保健營養學研究所/碩士(2004年)

摘要

本研究的目的在探討高分子水溶性幾丁聚醣對於免疫機能調節的功效 。選用健康的BALB/c雄鼠為動物模式，隨機分為4組，於小鼠週齡六週時 ，分別餵以正常飲食 (控制組)，再添加低、中、高不同劑量的幾丁聚醣於飼料中，餵食1個月後，將老鼠犧牲取其脾臟細胞及血液進行分析。分析的項目包括：脾臟中淋巴細胞的增殖反應、細胞激素分泌量以及淋巴細胞表面標記、分析血液中多核球及單核球的吞噬能力、血清中抗體濃度。實驗結果顯示，在淋巴細胞增殖方面，給予定量的裂殖素刺激後，在T淋巴球，低劑量組及高劑量組的增生能力皆顯著高於控制組；而B淋巴球方面，低劑量組及中劑量組的增生能力則低於控制組。細胞激素IFN-γ的分泌在高劑量組顯著的高於控制組。在淋巴細胞表面標記，自然殺手細胞(NK cell)在高劑量組時顯著的高於控制組。在吞噬細胞活性測定，顆粒球的測定其具有吞噬能力的細胞及對於E.coli的吞噬活性高劑量組顯著高於控制組。抗體分泌方面，IgG與IgM在高劑量組的分泌量顯著的高於控制組。整體而言，水溶性幾丁聚醣具有調節體內免疫反應的功能，而在高劑量組時，對於免疫的調節是最佳的。

關鍵字

幾丁聚醣 ； 免疫反應

並列摘要

The present study was to investigate effects of high molecular weight water soluble chitosan ( WSC ) on immune functions in BALB/c mice. In this study, normal male BALB/c mice aged six weeks were fed with basal diet which add different amounts of WSC depend on body weight of mices. After 1 month experimental treatment, BALB/c mices were sacrificed to evaluate immune responses by judging from lymphocyte proliferation, cytokines, subset of lymphocyte, phagocytic activity, and antibody analysis .The results show WSC could increase T lymphocyte proliferation in high-dose group.In the analysis of cytokines were secreted by splenocyte by using conA —stimulating, IFN-γ which was secreted in high-dose group significantly higher than control group. In determination of subset of lymphocyte, the amount of NK cell in high-dose group is higher than control group. The phagocytic activity of granulocyte in high-dose group were significantly higher than in control group. Secretion of IgG and IgM were increased in high-dose group. In conclusion , WSC can modulate immune response and high-dose WSC with significantl enhancing effect.

並列關鍵字

High molecular weight water soluble chitosan ； Immune response

參考文獻

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