台灣澤蘭活性成分之研究

台灣澤蘭地上部之丙酮/甲醇 (1:1) 萃取物，經由各種層析管柱及不同溶媒系統層析後，共分離出十八個化合物，其結構根據物理數據、核磁共振光譜分析後，共分為七大類：三個黃酮類化合物1 (Eupafolin)、2 (Hispidulin)、3 (Quercetin)；二個酚類化合物4 (Caffeic acid)、5 (Ethyl caffeoate)；一個三?類化合物6 (3?-Friedelinol)；一個二?類化合物7 (trans-Phytol)；二個固醇類化合物8 and 9 (?-sitosterol and? ?-stigmasterol)；一個??-內酯化合物10(3-ethoxymethyl -5H-furan-2-one)；八個倍半?類化合物11 (Eupatocannabin A), 12 (Eupatocannabin B), 13 (Eupatocannabin C), 14 (Eupatocannabin D), 15 (Eupatocannabin E), 16 (Eupatocannabin F), 17 (Eupaformolactone B), 18 (Eucannabinolide)，其中化合物11-16為文獻上首次發現的新化合物。此外，生物活性檢測方面，試驗的十七個化合物對於MCF-7細胞毒性試驗，而在50 μg/ml濃度下化合物1、11、12、13、14、15、16、17和18皆有明顯抑制，進一步降低濃度10 μg/ml以化合物1之細胞毒性37.85 % 為最低，化合物11、12、13、14、15、16、17和18皆在41~54 % 不等。

關鍵字

台灣澤蘭；倍半?環內酯；人類乳癌細胞；細胞毒性

並列摘要

The aerial part of Eupatorium cannabinum L. was extracted with acetone /methanol (1:1). By means of various chromatographic methods, eighteen compounds were isolated and characterized. Their structures were elucidated to be Eupafolin (1) , Hispidulin (2) and Quercetin (3) (flavonoid) ; Caffeic acid (4) , Ethyl caffeoate (5) (phenolic) ; 3?-Friedelinol (6) (triterpene) ; trans-Phytol (7) (diterpene) ; ?-sitosterol (8) and? stigmasterol (9) (steroid) ; 3-ethoxymethyl- 5H-furan-2-one (10) (?-lactone) ; Eupatocannabin A (11) , Eupatocannabin B (12) , Eupatocannabin C (13) , Eupatocannabin D (14) , Eupatocannabin E (15) , Eupatocannabin F (16) , Eupaformolactone B (17) , Eucannabinolide (18) (sesquiterpene) , and the compounds 11-16 are new natural products. In addition, the cytotoxic activity of the isolated constituents against MCF-7 was evaluated at the concentrations 50 μg/ml and 10 μg/ml. Results showed that compounds 1, 11, 12, 13, 14, 15, 16, 17 and 18 had high cytotoxicity at the concentration 50 μg/ml. Compound 1 had the lowest cytotoxicity 37.85 %, and compounds 11, 12, 13, 14, 15, 16, 17 and 18 had cytotoxicity range 41~54 % at the concentration 10 μg/ml.

並列關鍵字

Eupatorium cannabinum L. ； sesquiterpene lactone ； human breast cancer cells ； MCF-7 ； cytotoxicity

參考文獻

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台灣澤蘭活性成分之研究

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