黃豆皂素粗萃取物對於1,2-dimethylhydrazine 誘導F344 大鼠結腸癌前期病變異常腺窩灶的影響及機制之探討

The effect and mechanism of extracted crude soybean saponins on 1,2-dimethylhydrazine-induced ACF in F344 rats colon cancer

郭宇薇(Yu-Wei Guo)

指導教授 ： 林士祥

臺北醫學大學/公共衛生暨營養學院/保健營養學研究所/碩士(2007年)

https://doi.org/10.6831/TMU.2007.00042

摘要

本研究目的為探討黃豆皂素粗萃取物對結腸癌前期病變異常腺窩灶 (aberrant crypt foci, ACF) 的影響，並探討黃豆皂素粗萃取物降低結腸癌發生的可能機制。以雄性Fisher 344鼠做為動物模式，實驗分成五組在飲食當中添加不同劑量黃豆皂素粗萃取物，給予實驗飲食後第5週，以腹腔注射方式給予致癌物1,2-dimethylhydrazine (DMH) 誘發老鼠結腸癌的發生。在第15週予以犧牲，取出結腸，觀察結腸黏膜上ACFs數量、環氧化酶-2 (cyclooxygenase-2, COX-2) 蛋白質表現量、前列腺素E2 (prostaglandins E2, PGE2) 含量及β-葡萄糖醛酸酵素 (β-glucuronidase) 活性。結果顯示飲食中黃豆皂素粗萃取物可降低結腸黏膜上ACFs數量及β-glucuronidase活性，但不會影響COX-2蛋白質表現量及PGE2含量。飲食中的黃豆皂素粗萃取物對於結腸癌前期病變ACFs確實有一抑制功效，其機制可能與降低β-glucuronidase活性相關，而與COX-2及PGE2發炎反應的路徑無關。

關鍵字

異常腺窩灶 ； 黃豆皂素 ； 環氧化酶前列腺素 ； β-葡萄糖醛酸酵素

並列摘要

This study investigated the effect of extracted crude soybean saponins on preneoplastic lesions, aberrant crypt foci (ACF), and the related inhibitory mechanism of extracted crude soybean saponins. Rats assigned into five groups according to different doses of extracted crude soybean saponins and received 1,2-dimethylhydrazine (DMH) injection in week 5. In week 15, all rats were sacrificed. The number of ACFs, the cyclooxygenase-2 (COX-2) protein expression, the level of prostaglandins E2 (PGE2) and the activity of β-glucuronidase were examed. Results revealed that the consumption of extracted crude soybean saponins decreased the number of ACFs and the activity of β-glucuronidase in rats, while did not affect the COX-2 protein expression and PGE2 level. In conclusion, extracted crude soybean saponins decreased the number and size of ACFs. The mechanism may be correlated to the activity of β-glucuronidase in colonic mucosa but not the COX-2 protein expression and PGE2 level.

並列關鍵字

aberrant crypt foci (ACF) ； soybean saponins ； prostaglandins (PGE2) ； cyclooxygenase (COX-2) ； β-glucuronidase

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