透過您的圖書館登入
IP:54.204.117.206
  • 學位論文

給予β-glucan和Lactobacillus plantarum對高脂飼料誘發肥胖大鼠體重及體脂肪堆積之影響

Effects of ??-Glucan and/or Lactobacillus plantarum on Body Weightand Adipose Tissue in Obese Rats

指導教授 : 黃士懿

摘要


研究指出,大腸中短鏈脂肪酸(short-chain fatty acids, SCFAs)的量及濃度與肥胖有高度相關性。然而,腸道菌可利用益生質(如β-glucan)發酵產生SCFAs,SCFAs是否藉由影響脂肪生合成作用進而影響體重及體脂肪之研究尚未明確。因此本研究主要探討,以高脂飲食誘發肥胖之大鼠介入β-glucan或/和Lactobacillus plantarum對於體重及體脂肪的影響。實驗設計使用70隻雄性Sprague-Dawley大鼠分為正常飲食組及高脂飲食組,高脂飲食組誘發肥胖後再隨機分為6組,分別為控制組(control, C)、介入五倍劑量大麥β-glucan組(B)、Lactobacillus plantarum組(P)或合併給予Lactobacillus plantarum加一倍、二倍、五倍劑量的β-glucan (1X、2X、5X),實驗期為8週。結果發現β-glucan組和五倍劑量合併組可顯著降低體重增加量,而Lactobacillus plantarum組則有體重增加的趨勢,但各組體脂肪堆積及直腸糞便的腸道菌相均無顯著差異。五倍劑量合併組的攝食量顯著低於控制組。β-glucan組和五倍劑量合併組的盲腸和直腸糞便中的總SCFAs量顯著高於控制組,但大腸黏膜細胞中的總SCFAs無顯著增加,而肝臟酵素acetyl CoA carboxylase (ACC)的活性顯著增加,且在β-glucan組肝臟酵素fatty acid synthase (FAS)的活性顯著高於控制組。然而,Lactobacillus plantarum組在盲腸糞便的總SCFAs濃度及ACC和FAS酵素活性與控制組比較有上升趨勢,且直腸糞便和大腸黏膜細胞中的總SCFAs濃度與控制組相比有顯著增加。綜合上述結果發現,在高脂飲食下介入β-glucan可減緩體重的增加,主要原因可能是攝食量的降低。另外,飲食中添加Lactobacillus plantarum則可顯著增加大腸黏膜細胞中短鏈脂肪酸的濃度,進而誘發脂肪生合成作用,因此具有增加體重及體脂肪堆積的效力,而合併給予則無法發生加乘的效應。

並列摘要


The levels of short-chain fatty acids (SCFAs, i.e. acetate, propionate and butyrate) in the colon is highly associated with obesity. The impact of SCFAs, which produced by microorganisms, on body weight and white adipose tissue still remains unclear. The aim of the present study was to elucidate the barley β-glucan and/or Lactobacillus plantarum in body weight and white adipose tissue in diet-induced obesity rat. Seventy Sprague-Dawley rats were fed with normal diet or high fat diet to induce obesity and then rats were supplemented barley β-glucan (B), Lactobacillus plantarum (P), or both ingredients (Lactobacillus plantarum plus 1X, 2X, 5X β-glucan) for 8 weeks. In results, the diet intervention did not affect body weight, white adipose tissue and microbiota in distal fecal. But the levels of acetate and propionate significantly increased in cecum and distal colon of B and 5X groups. Moreover, the hepatic acetyl CoA carboxylase (ACC) activity was increased as well as the hepatic fatty acid synthase (FAS) activity in B group. SCFAs concentration and hepatic FAS and ACC activities showed no effect in P group. In conclusion, the rats fed with β-glucan and/or Lactobacillus plantarum lack the evidence of SCFAs on the reducing the body weight and white adipose tissue under high fat diet.

參考文獻


行政院衛生署 (2011). 100年度死因統計.
行政院衛生署國家衛生研究院 (2010). 2005-2008 國人過重及肥胖狀況.
Alfieri, M.A., Pomerleau, J., Grace, D.M., and Anderson, L. (1995). Fiber intake of normal weight, moderately obese and severely obese subjects. Obes Res 3, 541-547.
Arora, T., and Sharma, R. (2011). Fermentation potential of the gut microbiome: implications for energy homeostasis and weight management. Nutr Rev 69, 99-106.
Asahara, T., Nomoto, K., Shimizu, K., Watanuki, M., and Tanaka, R. (2001). Increased resistance of mice to Salmonella enterica serovar Typhimurium infection by synbiotic administration of Bifidobacteria and transgalactosylated oligosaccharides. J Appl Microbiol 91, 985-996.

延伸閱讀